DENV type 2 Nonstructural Protein 1 (DAG-P2500)

DENV type 2 Nonstructural Protein 1 (aa 776 - 1127), recombinant protein from HEK 293 cells

Product Overview
Dengue Virus 2 NS1 glycoprotein full length protein
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
40 kDa
Procedure
None
Purity
>95% by SDS-PAGE .This antigen is 0.2μm filter sterilised.
Format
Liquid
Buffer
pH: 7.40Constituent: 100% PBS
Preservative
None
Storage
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. pH: 7.40Constituent: 100% PBS
Introduction
The dengue virus (DENV) in one of five serotypes is the cause of dengue fever. It is a mosquito-borne single positive-stranded RNA virus of the family Flaviviridae; genus Flavivirus. All five serotypes can cause the full spectrum of disease.
Antigen Description
NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
Keywords
DENV-2 NS1

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References


Antibody Epitopes Identified in Critical Regions of Dengue Virus Nonstructural 1 Protein in Mouse Vaccination and Natural Human Infections

JOURNAL OF IMMUNOLOGY

Authors: Hertz, Tomer; Beatty, P. Robert; MacMillen, Zachary; Killingbeck, Sarah S.; Wang, Chunling; Harris, Eva

Dengue is a global public health problem and is caused by four dengue virus (DENV) serotypes (DENV1-4). A major challenge in dengue vaccine development is that cross-reactive anti-DENVAbs can be protective or potentially increase disease via Ab-dependent enhancement. DENV nonstructural protein 1 (NS1) has long been considered a vaccine candidate as it avoids Ab-dependent enhancement. In this study, we evaluated survival to challenge in a lethal DENV vascular leak model in mice immunized with NS1 combined with aluminum and magnesium hydroxide, monophosphoryl lipid A + AddaVax, or Sigma adjuvant system+CpG DNA, compared with mice infected with a sublethal dose of DENV2 and mice immunized with OVA (negative control). We characterized Ab responses to DENV1, 2, and 3 NS1 using an Ag microarray tiled with 20-mer peptides overlapping by 15 aa and identified five regions of DENV NS1 with significant levels of Ab reactivity in the NS1 + monophosphoryl lipid A + AddaVax group. Additionally, we profiled the Ab responses to NS1 of humans naturally infected with DENV2 or DENV3 in serum samples from Nicaragua collected at acute, convalescent, and 12-mo timepoints. One region in the wing domain of NS1 was immunodominant in both mouse vaccination and human infection studies, and two regions were identified only in NS1-immunized mice; thus, vaccination can generate Abs to regions that are not targeted in natural infection and could provide additional protection against lethal DENV infection. Overall, we identified a small number of immunodominant regions, which were in functionally important locations on the DENV NS1 protein and are potential correlates of protection.

Diagnostic parameters and reliability of four rapid immunochromatographic tests for dengue 4

BRAZILIAN JOURNAL OF INFECTIOUS DISEASES

Authors: Mata, Veronica Elizabeth; Lambert Passos, Sonia Regina; Borges dos Santos, Maria Angelica; Buonora, Sibelle Nogueira; Ferreira de Andrade, Carlos Augusto; Queiroz Lima, Monique da Rocha; Costa, Betina Moreira; Marques Hokerberg, Yara Hahr

Background: Although performance of rapid immunochromatographic tests (RITs) for dengue virus (DENY) serotypes 1, 2 and 3 is relatively settled, evidence on accuracy of RITs for DENV-4 are based on studies with small sample sizes and with discrepant results. Objectives: To assess accuracy and inter-observer agreement of RITs targeting dengue nonstructural protein-1 (NS1) antigen - Dengue NS1-Bioeasy (TM), Dengue NS1 Ag Strip-Bio-Rad (TM), IVB Dengue Ag NS1-Orangelife (TM) and Dengue NS1-K130-Bioclin (TM) in DENV-4 samples. Methods: Study sample (n = 324) included adults presenting at an emergency unit in Rio de Janeiro, Brazil, with fever of <= 72 h and two or more dengue symptoms. A serum sample from each patient was tested by each RIT. A positive reverse-transcription polymerase chain reaction was considered as the reference standard for dengue diagnosis. The diagnostic parameters analyzed for each RIT were sensitivity, specificity, positive and negative predictive values, and likelihood ratios. Each RIT was read by homogeneous (two junior nurses) or heterogeneous (one junior nurse and one senior biologist) pairs. Agreement was estimated by simple kappa with 95% confidence interval, positive (Ppos) and negative (Pneg) proportion concordance and prevalence and bias adjusted kappa, rated from poor (k < 0.0) to almost perfect (0.8 < k < 1.0), and perfect (k= 1). Results: NS1 RITs for DENV-4 diagnosis showed high specificity (95.9%-99.4%), but low sensitivity (14.7%-45.4%). BioeasyTM had the best performance, with a positive likelihood ratio of 26.0 (95% CI: 8.4;81.0). Inter-observer agreement was almost perfect for all evaluated RITs. Mismatches in confirmed dengue were more common for the BioclinTM (Ppos 88.3-90.0 %) and Orangelife (TM) (Ppos 91.7-94.1 %) tests. Conclusions: For DENV-4, the tested RITs had high specificity, but lower sensitivity compared to published results for other serotypes. They should not be used for screening purposes. Different brands may have very different performances. This should be considered upon deciding of using RITs in DENV-4 outbreaks. (C) 2020 Sociedade Brasileira de Infectologia. Published by Elsevier Espana, S.L.U.

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