Dengue NS1 Antigen ELISA kit (DEIABL14)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
Enzyme Immunoasay for the Determination of Dengue NS1 antigen level in Human Serum/Plasma.
Contents of Kit
1. Anti NS1 Dengue coated wells: 1 plate, 96 wells
2. Sample diluent: 1 vial of ready to use solution, 6 ml
3. Enzyme conjugate: 1vial, 12 ml
4. Positve control serum: 1 vial, 12 ml , Red color solution
5. Negative control serum: 1 vial, 3ml, Yelow color solution
6. Wash solution: 2 vials, 50 ml
7. Chromogen substrate reagent: 1 vial, 12 ml
8. Stop solution: 1 vial ,12 ml
9. Cardboard sealer
Storage
4°C
Precision
Intra-Assay: CV<14.7%
Inter-Assay: CV<13.8%
Sensitivity
99.21%

Citations


Have you cited DEIABL14 in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


The beta(4) subunit of Ca(v)1.2 channels is required for an optimal interferon response in cardiac muscle cells

SCIENCE SIGNALING

Authors: Tammineni, Eshwar R.; Carrillo, Elba D.; Soto-Acosta, Ruben; Angel-Ambrocio, Antonio H.; Garcia, Maria C.; Bautista-Carbajal, Patricia; del Angel, Rosa M.; Sanchez, Jorge A.

The auxiliary beta(4) subunit of the cardiac Ca(v)1.2 channel plays a poorly understood role in gene transcription. Here, we characterized the regulatory effects of the beta(4) subunit in H9c2 rat cardiac cells on the abundances of Ifnb mRNA [which encodes interferon-beta (IFN-beta)] and of the IFN-beta-related genes Ddx58, Ifitm3, Irf7, Stat2, Ifih1, and Mx1, as well as on the abundances of the antiviral proteins DDX58, IRF7, STAT2, and IFITM3. Knocking down the beta(4) subunit in H9c2 cells reduced the expression of IFN-beta-stimulated genes. In response to inhibition of the kinase JAK1, the abundances of beta(4) subunit mRNA and protein were decreased. beta(4) subunit abundance was increased, and it translocated to the nucleus, in cells treated with IFN-beta, infected with dengue virus (DENV), or transfected with poly(I:C), a synthetic analog of double-stranded RNA. Cells that surrounded the virus-infected cells showed translocation of beta(4) subunit proteins to nuclei in response to spreading infection. We showed that the beta(4) subunit interacted with the transcriptional regulator IRF7 and that the activity of an Irf7 promoter-driven reporter was increased in cells overexpressing the beta(4) subunit. Last, overexpressing beta(4) in undifferentiated and differentiated H9c2 cells reduced DENV infection and decreased the abundance of the viral proteins NS1, NS3, and E-protein. DENV infection and poly(I:C) also increased the concentration of intracellular Ca2+ in these cells. These findings suggest that the beta(4) subunit plays a role in promoting the expression of IFN-related genes, thereby reducing viral infection.

Enhancing the sensitivity of Dengue virus serotype detection by RT-PCR among infected children in India

JOURNAL OF VIROLOGICAL METHODS

Authors: Ahamed, Syed Fazil; Vivek, Rosario; Kotabagi, Shalini; Nayak, Kaustuv; Chandele, Anmol; Kaja, Murali-Krishna; Shet, Anita

Dengue surveillance relies on reverse transcription-polymerase chain reaction (RT-PCR), for confirmation of dengue virus (DENV) serotypes. We compared efficacies of published and modified primer sets targeting envelope (Env) and capsid-premembrane (C-prM) genes for detection of circulating DENV serotypes in southern India. Acute samples from children with clinically-diagnosed dengue were used for RT-PCR testing. All samples were also subjected to dengue serology (NS1 antigen and anti-dengue-IgM/IgG rapid immunochromatographic assay). Nested RT-PCR was performed on viral RNA using three methods targeting 654 bp C-prM, 511 bp C-prM and 641 bp Env regions, respectively. RT-PCR-positive samples were validated by population sequencing. Among 171 children with suspected dengue, 121 were dengue serology-positive and 50 were dengue serology-negative. Among 121 serology-positives, RT-PCR detected 91 (75.2%) by CprM654, 72 (59.5%) by CprM511, and 74 (61.1%) by Env641. Among 50 serology negatives, 10 (20.0%) were detected by CprM654,12 (24.0%) by CprM511, and 11 (22.0%) by Env641. Overall detection rate using three methods sequentially was 82.6% (100/121) among serology-positive and 40.0% (20/50) among serology-negative samples; 6.6% (8/120) had co-infection with multiple DENV serotypes. We conclude that detection of acute dengue was enhanced by a modified RT-PCR method targeting the 654 bp C-prM region, and further improved by using all three methods sequentially. (C) 2017 Elsevier B.V. All rights reserved.

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:

Related Products

Related Resources

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

Inquiry Basket