Bovine DNA Topoisomerase I, natural protein
Native bovine DNA Topoisomerase I (Scl-70)
SDS-PAGE (purity > 70%); Western blot with Scleroderma positive patient sera.
0.5-0.8 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
2-8°C short term, -20°C long term
DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis.
In human tissues the DNA topoisomerase I is initially synthesized as a protein with a molecular weight of 100 kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the Scl-70 antigen has derived its name
topoisomerase (DNA) I; 11986; Ensembl:ENSG00000198900; DNA topoisomerase 1; type I DNA topoisomerase; 18.104.22.168; TOP1; TOPI