Human DNA Topoisomerase I [His], recombinant protein from Baculovirus
Recombinant human DNA Topoisomerase I (Scl-70), his-tagged
SDS-PAGE (purity > 80%); Western blot with i: Scleroderma patient sera; ii:monoclonal anti-hexa-His-tag antibody.
0.5-0.8 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
2-8°C short term, -20°C long term
DNA topoisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis
topoisomerase (DNA) I; 11986; Ensembl:ENSG00000198900; DNA topoisomerase 1; type I DNA topoisomerase; 18.104.22.168; TOP1; TOPI