DKK1 Mouse ELISA Kit (DEIA1441)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell culture supernatant, serum, plasma
Species Reactivity
Mouse
Intended Use
For the quantitative measurement of Mouse DKK1 concentrations in serum, plasma and cell culture supernatants.
Contents of Kit
1. DKK1 Microplate
2. Wash Buffer Concentrate (20X)
3. Standards
4. Assay Diluent A
5. Assay Diluent B
6. Detection Antibody DKK1
7. HRP-Streptavidin Concentrate
8. TMB One-Step Substrate Reagent
9. Stop Solution
Storage
The DKK1 Mouse ELISA kit may be stored for up to 6 months at 2-8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Precision
Intra-assay: CV<10%
Inter-assay: CV<12%
Detection Range
8.23 pg/mL-6000 pg/mL
Sensitivity
25 pg/mL
Standard Curve

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References


Katoh, M; Katoh, M; et al. WNT signaling pathway and stem cell signaling network. CLINICAL CANCER RESEARCH 13:4042-4045(2007).
Yaccoby, S; Ling, W; et al. Antibody-based inhibition of DKK1 suppresses tumor-induced bone resorption and multiple myeloma growth in vivo. BLOOD 109:2106-2111(2007).

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