DIG-Detection PCR ELISA Kit

Sample

DIG-labeled products

Detection Method

cELISA

Intended Use

This DIG-Detection PCR ELISA, 5-pack kit is used for the qualitative or quantitative detection of DIG-labeled PCR products. It is particularly suitable for the parallel testing of a large number of samples.

Contents of Kit

Storage

When stored at +2 to +8°C, the kit is stable through the expiry date printed on the label.

Sensitivity

The sensitivity of the PCR ELISA technology is generally about 10 to 100-fold above that of the conventional analysis of PCR products in ethidium bromide stained agarose gels.
• 10 ng of genomic DNA as PCR template is usually enough for the detection of single-copy sequences in total human genomic DNA.
• If amplifying plasmid DNA or other less complex DNA templates, femtogram or picogram amounts of DNA can usually be detected.

Reconstitution And Storage

Content:
Anti-DIG-POD (Vial 5)
Preparation:
Add 250 μl PCR-grade water* to one vial of lyophilizate; mix carefully. Do not vortex.
Let sit 15 minutes at +15 to +25°C.
The lyophilizate should be a clear solution after reconstitution. Any particles within the reconstituted solution are to be considered as deterioration.
Storage and Stability
Store 2 months at +2 to +8°C. Do not freeze.
For use in Anti-DIG-POD working solution.