Greater than 95% as determined by SDS-PAGE.
The Dengue Envelope 2 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Envelope protein E takes part in membrane fusion between virion and host late endosomes. Virus Envelope 2 is synthesized as a homodimer with prM which plays a role as a chaperone for envelope protein E. Envelope protein E departs from small envelope protein M and homodimerizes after cleavage of prM. Recombinant Dengue Envelope 2 produced in E. coli is a single polypeptide chain containing 126 amino acids (aa 298-400) and having a molecular mass of 13.8kDa. Recombinant Dengue Envelope 2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Membrane glycoprotein precursor; Dengue virus 2; Flavivirus; DENV; NS1 Protein; Non Structural Protein; Dengue Type 2; Dengue Type 2; DEN2; Dengue NS1; Dengue Virus NS1