DENV Polyvalent Antigen (DAGA-095)

Nature
Recombinant
Tag/Conjugate
Unconjugated
Molecular Weight
22kDa
Procedure
None
Purity
Protein is>95% pure as determined by 12% PAGE (coomassie staining).
Format
Solution
Size
100µg; 500µg; 1000µg
Buffer
Phosphate buffered saline, pH-7.4 and 0.05% NaN3.
Preservative
0.05% Sodium Azide
Storage
Stored below -18°C.
Stability
Stable at 4°C for 1 week. Please prevent freeze thaw cycles.
Ship
Ice Packs
Antigen Description
Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholino antisense oligos have shown specific activity against Dengue virus. Polyvalent dengue antigen is a 22kDa protein that contains 4 dengue antigen subtypes, produced in E.coli and fused to a 6xHis Tag. The immunoreactive peptide span from the viral envelope to the non-functional region. The polyvalent dengue antigen consists of 25% recombinant antigen from each of the dengue subtypes in their composition. The Polyvalent dengue antigen detects all four dengue subtypes. The sensitivity and specificity could reach approximately 93% and 96% in dengue IgG and IgM ELISA assay.
Keywords
Membrane glycoprotein precursor; Dengue virus; Flavivirus; DENV

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