Dab1 (Phospho-Tyr232) ELISA Kit

Sample

Cultured cells

Species Reactivity

Human; Mouse; Rat

Detection Method

Indirect ELISA

Intended Use

The Dab1 (Phospho-Tyr232) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Dab1 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Dab1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Dab1 phosphorylation.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 30 mL (10x)
3. Quenching Buffer: 24 mL (1x)
4. Blocking Buffer: 50 mL (1x)
5. 15x Wash Buffer: 60 mL (10x)
6. 100x Anti-Dab1 (Phospho-Tyr232) Antibody (Rabbit Polyclonal): 60 μL (100x)
7. 100x Anti-Dab1 Antibody (Rabbit Polyclonal): 60 μL (100x)
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x)
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x)
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x)
11. Primary Antibody Diluent: 12 mL (1x)
12. Ready-to-Use Substrate: 12 mL (1x)
13. Stop Solution: 12 mL (1x)
14. Crystal Violet Solution: 12 mL (1x)
15. SDS Solution: 24 mL (1x)
16. Adhesive Plate Seals: 2 seals

Storage

4°C/6 Months