Cotinine (Nicotine) ELISA Kit (DEIA6844)

Regulatory status: For research use only, not for use in diagnostic procedures.

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water, urine, saliva samples
Species Reactivity
Intended Use
The Cotinine ELISA is an immunoassay for the quantitative and sensitive detection of Cotinine. This test is suitable for the quantitative and/or qualitative screening of Cotinine in water, urine, and saliva samples. If necessary, positive samples can be confirmed by HPLC, GC/MS, or other conventional methods.
Contents of Kit
1. Microtiter plate
2. Standards (6)
3. Antibody Solution, 6 mL
4. Cotinine-HRP Conjugate Solution, 6 mL
5. Diluent/zero, 25 mL
6. Wash Solution (5X) Concentrate, 100 mL
7. Color Solution (TMB), 12 mL
8. Stop Solution, 12 mL
The Cotinine ELISA should be stored in the refrigerator (4-8°C). The solutions must be allowed to reach room temperature (20-25°C) before use. For more detailed information, please download the following document on our website.
The estimated minimum detectable concentration, based on 90% B/Bo, is 0.045 ppb (μg/L) in water, 4.5 ppb (μg/L) in urine, and 9.0 ppb (μg/L) in saliva.


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