Loading control proteins are well-known for their constitutive and stable expressions at high levels in almost all tissues and cells. The expression levels of loading controls in different tissues and cells are comparatively equivalent. Therefore they are important in evaluating whether protein loading in each well is identical across a gel. When choosing a loading control, it should be considered that the chosen loading control must have a different molecular weight to that of the protein of your interest, avoiding overlapping staining and interference to the result.
Creative Diagnostics offers highly specific antibodies to the most common targets, such as: β-Actin, COX IV, GAPDH, Histone H3, and α- and β-Tubulin. Our antibodies are manufactured under extensive quality control to ensure optimal sensitivity and consistency.