Clenbuterol ELISA Kit

Name: Clenbuterol ELISA Kit
SKU: DEIA016
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Urine, liver, feed, tissue and meat

Detection Method

cELISA

Intended Use

This test kit is based on the competitive enzyme immunoassay for the detection of Clenbuterol in the sample.

Contents of Kit

Note: ppb=μg/kg
1. Standard working solutions: 6 bottles, 1.5 mL each, with concentrations of 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, and 8.1 ppb.
2. Microplate: 1 plate (8 wells×12 strips)
3. Conjugate concentrate: 1 bottle (11×, 1 mL)
4. Conjugate diluent: 1 bottle (9 mL)
5. Concentrated wash solution: 1 bottle (20×, 25 mL)
6. Concentrated tissue diluent: 1 bottle (50×, 30 mL)
7. Substrate A and B solutions: 1 bottle each (7 mL)
8. Stop solution: 1 bottle (7 mL)
9. Instructions
10. Plate cover film
11. Sealed bags

Storage

Store the kit at 2-8°C, avoiding freezing, with a shelf life of 1 year. Unused microplate strips should be sealed and stored at 2-8°C°C.

Performance Characteristics

Sensitivity: 0.1 ppb
Standard curve range: 0.1 ppb-8.1 ppb
B0 absorbance should be above 0.8.
Intra-plate error <5%, inter-plate error <10%.
Recovery rate with the provided method: 90%±30%.
Minimum detection limits:
Swine urine, pork, beef, mutton, chicken, duck, swine liver, processed meat, rabbit: approx. 0.5 ppb
Bovine and ovine urine: approx. 1 ppb
Feed: approx. 5 ppb
Note: ppb=μg/kg

General Description

Clenbuterol, a β-agonist drug,was commonly added to animal feed to increase the lean meat ratio. However, its consumption through animal products can cause various human health issues, leading to a global ban. This kit offers a convenient, rapid, and sensitive method for detecting clenbuterol residues in a large number of samples.

Standard Curve

Note: Input absorbance values, standard concentrations, and dilution factors into the software for automatic curve fitting and result calculation. Environmental factors and operational differences may affect absorbance values and curve shape.
Analytical Limitations: ewf4This kit is for screening purposes only. Positive results should be confirmed with instrumental methods like GC/MS or LC-MS/MS.

Citations

Have you cited DEIA016 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

A novel murine antibody and an open sandwich immunoassay for the detection of clenbuterol

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

Authors: Cong, Yang; Dong, Hang; Wei, Xiaoyuan; Zhang, Liqian; Bai, Jingkun; Wu, Jingliang; Huang, Johnny X.; Gao, Zhiqin; Ueda, Hiroshi; Dong, Jinhua

Abstract

Clenbuterol (CLEN) is a sympathomimetic amine used as a decongestant and bronchodilator while treating breathing disorders. It is also used in food-producing animals as it improves the rate of red meat production. However, it is prohibited in many countries nowadays due to human health and safety concerns. Unfortunately, the illegal use of CLEN is still rampant. Thus, monitoring it in food and livestock is important. Here, we report a novel murine antibody and an open sandwich enzyme linked immunosorbent assay (OS-ELISA) to detect CLEN based on antigen-antibody reactions. The genes of antibody variable regions in mice immunized with CLEN conjugated with bovine serum albumin were cloned into a phagemid (pDong1/Fab) to construct a phage-display antibody library, from which a novel antibody, A12, was selected. Then, an OS-ELISA was developed to detect CLEN using separated variable regions of the A12 antibody. The limit of detection of the assay was found to be 8 ng/mL, which was useful for monitoring CLEN usage.

Heritability of primocane-yield components in red raspberry

XI INTERNATIONAL RUBUS AND RIBES SYMPOSIUM

Authors: Gonzalez, M. D.

Abstract

A partial diallel design with 21 F-1 sib-families and their reciprocal families was planted in a randomized complete block design in May 2014 in Oxnard, CA, USA and the primocane crop evaluated in the fall of 2014. Average marketable (GYLD) and non marketable (BYLD) fruit yield, cane number (NCAN), cane height (CLEN), cane diameter (CDIAM), number of nodes (NBUD), number of laterals (NLAT), lateral length (LLEN), number of fruits per lateral (NFRT), and berry weight (BWT) was recorded for 2 individual plants within each family throughout the course of the entire study. Narrow-sense heritability, genotypic and phenotypic correlations, and general (GCA) and specific (SCA) combining ability, and reciprocal effects were estimated. Narrow sense heritability estimates ranged from moderately low (GYLD=0.27) to moderately high (NCAN=0.70). GYLD had a strong positive genetic correlation with CLEN (r(a)=+0.73), moderate correlation with NBUD (r(a)=+0.49), and poor correlation with NLAT (r(a)=+0.14), LLEN (r(a)=+0.11), BYLD (r(a)=+0.23), and BWT (r(a)=+0.03). A negative genetic correlation was observed with NCAN (r(a)=-0.34) and CDIAM (r(a)=-0.04). GCA effects were significant for all traits except for CDIAM, BWT, and GYLD. SCA and reciprocal effects were also significant for all traits except for CDIAM and BWT.