A novel murine antibody and an open sandwich immunoassay for the detection of clenbuterol
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Authors: Cong, Yang; Dong, Hang; Wei, Xiaoyuan; Zhang, Liqian; Bai, Jingkun; Wu, Jingliang; Huang, Johnny X.; Gao, Zhiqin; Ueda, Hiroshi; Dong, Jinhua
Clenbuterol (CLEN) is a sympathomimetic amine used as a decongestant and bronchodilator while treating breathing disorders. It is also used in food-producing animals as it improves the rate of red meat production. However, it is prohibited in many countries nowadays due to human health and safety concerns. Unfortunately, the illegal use of CLEN is still rampant. Thus, monitoring it in food and livestock is important. Here, we report a novel murine antibody and an open sandwich enzyme linked immunosorbent assay (OS-ELISA) to detect CLEN based on antigen-antibody reactions. The genes of antibody variable regions in mice immunized with CLEN conjugated with bovine serum albumin were cloned into a phagemid (pDong1/Fab) to construct a phage-display antibody library, from which a novel antibody, A12, was selected. Then, an OS-ELISA was developed to detect CLEN using separated variable regions of the A12 antibody. The limit of detection of the assay was found to be 8 ng/mL, which was useful for monitoring CLEN usage.
Heritability of primocane-yield components in red raspberry
XI INTERNATIONAL RUBUS AND RIBES SYMPOSIUM
Authors: Gonzalez, M. D.
A partial diallel design with 21 F-1 sib-families and their reciprocal families was planted in a randomized complete block design in May 2014 in Oxnard, CA, USA and the primocane crop evaluated in the fall of 2014. Average marketable (GYLD) and non marketable (BYLD) fruit yield, cane number (NCAN), cane height (CLEN), cane diameter (CDIAM), number of nodes (NBUD), number of laterals (NLAT), lateral length (LLEN), number of fruits per lateral (NFRT), and berry weight (BWT) was recorded for 2 individual plants within each family throughout the course of the entire study. Narrow-sense heritability, genotypic and phenotypic correlations, and general (GCA) and specific (SCA) combining ability, and reciprocal effects were estimated. Narrow sense heritability estimates ranged from moderately low (GYLD=0.27) to moderately high (NCAN=0.70). GYLD had a strong positive genetic correlation with CLEN (r(a)=+0.73), moderate correlation with NBUD (r(a)=+0.49), and poor correlation with NLAT (r(a)=+0.14), LLEN (r(a)=+0.11), BYLD (r(a)=+0.23), and BWT (r(a)=+0.03). A negative genetic correlation was observed with NCAN (r(a)=-0.34) and CDIAM (r(a)=-0.04). GCA effects were significant for all traits except for CDIAM, BWT, and GYLD. SCA and reciprocal effects were also significant for all traits except for CDIAM and BWT.