Indirect ELISA IC50: 0.5 ppb Matched pair conjugate available for Ciprofloxacin antibody: Ciprofloxacin [BSA](Catalog # DAGA-058B) Ciprofloxacin [HRP](Catalog # DAGA-046H)
Target
Alternative Names
Ciprofloxacin; Ciprobay
Citations
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Simple, fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensor
Rafaela Silva Lamarca, Ricardo Adriano Dorledo de Faria, Maria Valnice Boldrin Zanoni, Marcelo Nalin, Paulo Clairmont Feitosa de Lima Gomes, Younès Messaddeq
Applications: ELISA Reactive species: Unspecified reactive species
"Abstract: In this study an impedimetric immunosensor was developed in order to determine ciprofloxacin (CIP) in wastewater samples, an emergent contaminant widely found in wastewater. To achieve this, an anti-ciprofloxacin antibody was immobilized on the surface of a printed carbon electrode. Then, the developed immunosensor was applied in wastewater samples from Université Laval residences (Québec, Canada) through the load transfer resistance (Rct) using [Fe(CN)6]3−/4− as a redox probe, and the average CIP concentration was found to be 2.90 × 10−4 μg mL−1. The observed Rct changes presented a linear relationship from CIP concentrations of 10−5 to 1.0 μg mL−1, with detection and quantification limits of 2.50 × 10−6 and 7.90 × 10−6 μg mL−1, respectively. The immunosensor presented high selectivity and repeatability, as well as a good recovery rate in wastewater samples (97%). Significant interference with other compounds was not observed. The proposed method requires only 30 μL of sample without the use of organic solvents or preceding sample preparation and/or extraction techniques. Moreover, the method is fast: only 20 min of incubation followed by 2 min of analysis time was sufficient to obtain the CIP concentration. The method's estimated cost is U$ 2.00 per sample." Article snippet: The anti-ciprofloxacin monoclonal antibody (IgG) (1 mg mL-1) was purchased from Creative Diagnostics.
Figure 1. Scheme of SPCE surface modification for ciprofloxacin determination.
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