Contents of Kit
1. Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96), 1 frame, the coating material is inactivated, 12
2. Standard serum (ready-to-use), Human serum in phosphate buffer with protein; negative for antiHIV-Ab, anti-HBs-Ag (Hepatitis B-Virus-surface antigen) and anti HCV-Ab; preservative: < 0.1 % sodium azide, coloring: Amaranth O. 2 × 2 ml
3. Negative control serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV, anti-HBs (Hepatitis B-Virus-surface antigen) and anti-HCV; preservative: < 0.1 % sodium azide, coloring: Lissamin green V . 2 ml
4. Anti-human-IgG-conjugate (ready-to-use), Anti-human-IgG from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane.13 ml.
5. Washing solution concentrate (sufficient for 1 litre), Sodium chloride solution with Tween 20, 30 mM Tris, preservative: < 0.1 % sodium azide. 33.3 ml
6. Dilution buffer, Phosphate buffer with protein and Tween 20; preservative: < 0.1 % sodium azide, 0.01 g/l Bromphenol blue sodium salt. 2 × 50 ml
7. Stopping solution, 1.2 N sodium hydroxide. 15 ml
8. Substrate (ready-to-use), Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide. (Substrate in unopened bottle may have a slightly yellow coloring. This does not reduce the quality of the product!) 13 ml