Chlamydia Trachomatis IgG ELISA Kit

Sample

serum or plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Chlamydia Trachomatis IgG ELISA Kit is intended for the detection of IgG antibody to Chlamydia Trachomatis in human serum or plasma.

Contents of Kit

1. Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96), 1 frame, the coating material is inactivated, 12
2. Standard serum (ready-to-use), Human serum in phosphate buffer with protein; negative for antiHIV-Ab, anti-HBs-Ag (Hepatitis B-Virus-surface antigen) and anti HCV-Ab; preservative: < 0.1 % sodium azide, coloring: Amaranth O. 2 × 2 ml
3. Negative control serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV, anti-HBs (Hepatitis B-Virus-surface antigen) and anti-HCV; preservative: < 0.1 % sodium azide, coloring: Lissamin green V . 2 ml
4. Anti-human-IgG-conjugate (ready-to-use), Anti-human-IgG from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane.13 ml.
5. Washing solution concentrate (sufficient for 1 litre), Sodium chloride solution with Tween 20, 30 mM Tris, preservative: < 0.1 % sodium azide. 33.3 ml
6. Dilution buffer, Phosphate buffer with protein and Tween 20; preservative: < 0.1 % sodium azide, 0.01 g/l Bromphenol blue sodium salt. 2 × 50 ml
7. Stopping solution, 1.2 N sodium hydroxide. 15 ml
8. Substrate (ready-to-use), Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide. (Substrate in unopened bottle may have a slightly yellow coloring. This does not reduce the quality of the product!) 13 ml