The Chikungunya IgM-μ-capture ELISA is intended for measurement of IgM class antibodies to Chikungunya virus in human serum and plasma (citrate).
Contents of Kit
1. Chikungunya Microplate (IgM): 12 breakapart 8-well snap-off strips coated with anti human IgM, in resealable aluminium foil.
2. Sample Diluent ***: 1 bottle containing 100 mL of ready to use buffer for sample dilution; pH 7.2 ± 0.2; coloured yellow; white cap.
3. Stop Solution: 1 bottle containing 15 mL. Ready to use sulphuric acid, 0.2 mol/L; red cap.
4. Washing Solution (20x conc.)*: 1 bottle each containing 50 mL of a 20-fold concentrated buffer (pH 7.2 ± 0.2) for washing the wells; white cap.
5. Chikungunya antigen, lyophilized: 6 bottles containing lyophilized Chikungunya antigen solution; red cap
6. Chikungunya antibody Solution ****: 1 bottle containing 6 mL of biotinylated Chikungunya antibody, ready to use; coloured blue; white cap.
7. Streptavidin conjugate**: 1 bottle containing 6 mL Streptavidin conjugated with peroxidase, ready to use; coloured red; black cap.
8. TMB Substrate Solution: 1 bottle containing 15 mL 3,3',5,5'-tetramethylbenzidine (TMB); ready to use; yellow cap.
9. Chikungunya IgM High Control****: 1 bottle containing 1.5 mL; coloured yellow; ready to use; red cap.
10. Chikungunya IgM Calibrator Control****: 1 bottle containing 2 mL; coloured yellow; ready to use; green cap.
11. Chikungunya IgM Low Control***: 1 bottle containing 1.5 mL; coloured yellow; ready to use; blue cap.
* contains 0.1% Bronidox L after dilution
** contains 0.2% Bronidox L
*** contains 0.1% Kathon
**** contains 0.02% Kathon and 0.02% Bronidox L
12. 1 Strip holder
13. 1 Cover foils
14. 1 Test protocol
15. 1 distribution and identification plan
For more detailed information, please download the following document on our website.
Chikungunya virus is an arthropod borne virus of the genus Alphavirus (family Togaviridae). The Alphavirus genus contains at least 24 distinct species. These are lipid-enveloped virions with a diameter of 50 to 60 nm. Alphavirus infections are initiated by the bite of an infected mosquito, which results in the deposition of virus in subcutaneous and possibly cutaneous tissues. After an incubation period of 1 to 12 days the Chikungunya fever develops. Chikungunya fever (Chikungunya means "that which bends up", in reference to the crippling manifestations of the disease) is an acute viral infection characterized by a rapid transition from a state of good health to illness that includes severe arthralgia and fever.
The virus has major importance in Africa and Asia. From 20% to more than 90% of the population of tropical and subtropical show serologic evidence of infection. Because Aedes mosquitoes are increasingly prevalent in North Africa and South America, where the population would be uniformly susceptible to infection, the possibility for epidemics is evident. Chikungunya virus infections are imported to central Europe mainly by travellers to tropical and subtropical countries.