Efficacy of repeated ovum pick-up in Podolic cattle for preservation strategies: a pilot study
ITALIAN JOURNAL OF ANIMAL SCIENCE
Authors: Presicce, Giorgio Antonio; Neglia, Gianluca; Salzano, Angela; Padalino, Barbara; Longobardi, Valentina; Vecchio, Domenico; De Carle, Esterina; Gasparrini, Bianca
The study evaluated the effects of eCG treatment prior to ovum pick up (OPU) on follicular population, oocyte and embryo yields in summer and autumn in Podolic cattle. The effects of repeated OPU on cattle wellbeing was also documented. Twenty-six animals were used, and split into two groups, treatment (OPU; n = 18) and control (CG; n = 8). The OPU cattle were subsequently split into two subgroups (n = 9) and underwent repeated OPU, without and with eCG priming, for a total of 8 sessions (4 sessions/season). Follicular population, oocyte and embryo yields were recorded in those sub-groups. CG was handled in the same manner of OPU, except for epidural anaesthesia and follicular aspiration. Biochemical profile, serum protein electrophoresis and haptoglobin levels were analysed in OPU and CG. Hormonal priming increased the number of medium follicles (1.7 +/- 0.2 vs 0.9 +/- 0.2, p < .05), while it decreased the recovery rate and number of cumulus-enclosed oocytes (COCs) (recovery rate: 38.3 +/- 3.5 vs 60.5 +/- 4.0%; COCs: 2.3 +/- 0.3 vs 3.4 +/- 0.4, respectively; p < .01). However, priming increased cleavage (72.9 +/- 5.7 vs 49.4 +/- 5.4; p < .05) and blastocyst (41.1 +/- 5.7 vs 23.0 +/- 4.2; p = .054) rates. With regard to season's effect, a higher number of COCs was recorded in autumn than in summer (3.1 +/- 0.4 vs 2.6 +/- 0.3; p < .05) without affecting though the number of embryos produced (0.9 on average). Since haematological parameters did not vary between OPU and CG, our preliminary data suggest that repeated OPU may be used as a conservation strategy in Podolic cattle without affecting wellbeing.
Prozone phenomenon observed in indirect immunofluorescence assay by antibodies against neuronal antigens
JOURNAL OF NEUROIMMUNOLOGY
Authors: Mathai, Annamma; Panicker, Suprabha; Kannoth, Sudheeran; Anandakuttan, Anandkumar
A marked prozone effect was observed in indirect immunofluorescence with human sera and human cere-brospinal fluid in two clinical cases involving breast carcinoma with paraneoplastic neuronal antibodies, and anti N-methyl-D-aspartic acid (NMDA) receptor antibodies. Anti-Yo antibodies and anti-NMDA antibodies were not detectable under high concentrations (1:10 serum dilution and neat CSF respectively) but showed a true effect when sufficiently diluted at 1:80 and 1:5 respectively. This paper demonstrates that prozone effects have their occurrences in indirect immunofluorescence, and clinicians and laboratory technicians should be wary of its implications during screening of autoantibody markers in neurological diseases.