Chicken IgG (IgY) ELISA Kit

Name: Chicken IgG (IgY) ELISA Kit
SKU: DEIA3658
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Plasma, Serum

Species Reactivity

Chicken

Detection Method

sELISA

Intended Use

The IgG (IgY) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG (IgY) in chicken biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use.

Contents of Kit

1.ELISA Micro Plate, antibody coated. One plate of 12 removable 8 well strips, antibody coated.
2.Enzyme Conjugated Detection Antibody. One vial of 150μL of 100X Horseradish Peroxidase Conjugated antibody in a stabilizing buffer.
3.Calibrator. One vial of calibrator.
4.Diluent Concentrate. One 50 mL bottle of 5X diluent buffer.
5.Wash Solution Concentrate. One 50 mL bottle of 20X wash solution.
6.ChromogenSubstrate Solution. One bottle of 12 mL 3,3',5,5' tetramethylbenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
7.STOP Solution. One 12 mL bottle of 0.3 M sulfuric acid. WARNING: Avoid Contact with Skin.

Storage

Store the kit at 2-8°C.
1. ELISA Micro Plate, antibody coated. 4-8°C, In sealed foil bag with desiccant. With proper storage the plate strips are stable until the expiration date.
2. Enzyme Conjugated Detection Antibody. 4-8°C in the dark. The working conjugate solution should be diluted immediately prior to use. The 100X conjugate is stable until the expiration date.
3. Calibrator. 4-8°C for lyophilized calibrator. Aliquoted and frozen if reconstituted. Avoid multiple freeze-thaw cycles. The working standard solutions should be prepared immediately prior to use.
4. Diluent Concentrate. 4-8°C for both 1X working solution and 5X concentrate.
5. Wash Solution Concentrate. 4-8°C for both 1X working solution and 20X concentrate. The 1X working solution is stable for at least one week from the date of preparation. The 20X concentrate is stable until the expiration date.
6. STOP Solution. The Stop Solution is stable until the expiration date.

Detection Range

6.25 ng/ml - 400 ng/ml

Sensitivity

1.508 ng/ml

Standard Curve

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

Immunoassays for Process-related Impurities

QA & Reviews

Customer Reviews

References

Real-Time Nanoplasmonic Sensor for IgG Monitoring in Bioproduction

PROCESSES

Authors: Tran, Thuy; Eskilson, Olof; Mayer, Florian; Gustavsson, Robert; Selegard, Robert; Lundstrom, Ingemar; Mandenius, Carl-Fredrik; Martinsson, Erik; Aili, Daniel

Abstract

Real-time monitoring of product titers during process development and production of biotherapeutics facilitate implementation of quality-by-design principles and enable rapid bioprocess decision and optimization of the production process. Conventional analytical methods are generally performed offline/at-line and, therefore, are not capable of generating real-time data. In this study, a novel fiber optical nanoplasmonic sensor technology was explored for rapid IgG titer measurements. The sensor combines localized surface plasmon resonance transduction and robust single use Protein A-modified sensor chips, housed in a flexible flow cell, for specific IgG detection. The sensor requires small sample volumes (1-150 mu L) and shows a reproducibility and sensitivity comparable to Protein G high performance liquid chromatography-ultraviolet (HPLC-UV). The dynamic range of the sensor system can be tuned by varying the sample volume, which enables quantification of IgG samples ranging from 0.0015 to 10 mg/mL, without need for sample dilution. The sensor shows limited interference from the sample matrix and negligible unspecific protein binding. IgG titers can be rapidly determined in samples from filtered unpurified Chinese hamster ovary (CHO) cell cultures and show good correlation with enzyme-linked immunosorbent assay (ELISA).

Increased Risk of Toxoplasma gondii Infection in Patients with Colorectal Cancer in Eastern China: Seroprevalence, Risk Factors, and a Case-Control Study

BIOMED RESEARCH INTERNATIONAL

Authors: Yu, Yang; Guo, Dong; Qu, Tingting; Zhao, Shuchao; Xu, Chang; Wang, Longlong; Wang, Zhongjun; Fu, Haiyang; Zhang, Xiangyan; Zhou, Na

Abstract

The aim of this study was to explore the epidemiology ofToxoplasma gondiiinfection in patients with colorectal cancer (CRC) in eastern China. Therefore, 287 primary CRC patients and 287 age-matched healthy control subjects were recruited to estimate the seroprevalence ofT. gondiiand identify the risk factors of infection. Enzyme-linked immunoassays were used to test for anti-T. gondiiimmunoglobulin G (IgG) and IgM antibodies. Forty-six (16%) samples were positive for anti-T. gondiiIgG antibodies in patients with CRC, compared with 26 (9.1%) in the healthy controls, a significant difference (P=0.007). By contrast, eight (2.8%) patients tested positive forT. gondiiIgM antibodies, compared with three (1.1%) in the controls, a difference that was not significant (P=0.13). Multivariable backward stepwise logistic regression analysis revealed that a rural residence (OR 2.83; 95% CI 1.15-7.01;P=0.024) and treatment with chemotherapy (OR 2.16; 95% CI 1.02-4.57;P=0.045) were risk factors forT. gondiiinfection in patients with CRC. Thus,T. gondiiinfection is serious in patients with CRC, and a rural residence and treatment with chemotherapy are independent risk factors for infection by this parasite. Therefore, medical professionals should be aware of this pathogen in patients with CRC, and the causes ofT. gondiiinfection in these patients need to be explored further.