The Benomyl/Carbendazim ELISA is an immunoassay for the detection of Benomyl and Carbendazim in surface water. This test is suitable for the quantitative and/or qualitative detection of Benomyl and Carbendazim in contaminated samples. Positive samples should be confirmed by HPLC, GC/MS, or other conventional methods.
The Benomyl/Carbendazim ELISA Kit should to be stored in the refrigerator (4–8°C). The solutions must be allowed to reach room temperature (20-25°C) before use. Reagents may be used until the expiration date on the box. The conjugate is supplied in lyophilized form (3 vials). Before each assay, the required volume of lyophilized conjugate must be reconstituted (see Test Preparation section). Reconstitute only the amount needed for the samples to be run, as the reconstituted solution will only remain viable for one week (store frozen).
The limit of quantitation for Carbendazim (90% B/B0) is approximately 0.089 ng/mL. The concentration of residue necessary to cause 50% inhibition (50% B/B0) is approximately 0.687 ng/mL. Determinations closer to the middle of the calibration range of the test yield the most accurate results.