Cefalexin ELISA Kit (DEIANJ05)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
milk, tissue, honey, urine
Species Reactivity
Bovine
Intended Use
The Cefalexin ELISA is a competitive enzyme immunoassay based on specific antibodies directed against cefalexin.
Storage
Store at 2 - 8°C.
Detection Limit
Milk 5.0ppb
Tissue 6.5ppb
Honey 6.8ppb
Urine 3.4ppb
General Description
Cephalosporins are bactericidal beta-lactam antibiotics and have the same mode of action as other beta-lactam antibiotics such as penicillins. Cefalexin is a first generation cephalosporin which is used for infections caused by gram-positive bacteria. The EU has set MRLs for cefalexin: 100 µg/kg in bovine milk, 200 µg/kg in bovine muscle, fat and liver and 1000 µg/kg in bovine kidney.

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References


Survey on bacterial isolates from dogs with urinary tract infections and their in vitro sensitivity

REVUE DE MEDECINE VETERINAIRE

Authors: Papini, R; Ebani, VV; Cerri, D; Guidi, G

Bacterial isolates from urine specimen of dogs with urinary tract infections in a geographical region of Central Italy were : Escherichia coli (17.48%), Proteus mirabilis (16.59%), Pseudonionas aeruginosa (7.17%), Staphylococcus aureus (6.72%), alpha-haemolytic streptococci (2.24%), and Klebsiella sp. (0.89%). In vitro antibacterial agent sensitivity showed that none of them was effectively susceptible to any of the antibacterial agents tested, including amikacin, amoxicillin, amoxicillin-clavulanic acid, ampicillin, cefalexin, cefotaxin, doxicyclin, enrofloxacin, erythromycin, gentamycin, kanamycin, netilmycin, rifampicin, streptomycin, sulphamethoxazole/trimethoprim, and tetracyclin.

Factors influencing antimicrobial utilization at a university hospital during a period of 11 years

INTERNATIONAL JOURNAL OF CLINICAL PHARMACOLOGY AND THERAPEUTICS

Authors: Vlahovic-Palcevski, V; Palcevski, G; Mavric, Z; Francetic, I

Objective: A number of factors may influence antibiotic prescribing. The objective of this study was to identify trends in antimicrobial prescribing during a period of 11 years at the University Hospital Center Rijeka (UHC), Croatia, and to identify possible factors that might have influenced changes in prescribing patterns. This may help in establishing criteria for future intervention. Methods: Antimicrobial utilization was evaluated retrospectively for the 11-year period from 1990-2000. It was measured in defined daily doses (DDDs) per 100 bed days using the ATC Index with DDDs 2000. Results: During the investigation period, marked differences were noted in total and individual antimicrobial consumption. In the first 4 years of this study, the utilization of all groups of antimicrobials decreased, while in its second part (i.e. from 1995 - 1997) an increase in utilization of all antimicrobial groups occurred. Changes in utilization of groups of antimicrobials did not coincide completely with the changes in total antimicrobial utilization. The most prominent changes were a decrease in penicillin and cephalosporin utilization, and an increase of macrolides and aminoglycosides utilization from 1997 - 2000. Ampicillin and cefalexin were mostly prescribed as single antibiotics during a 5-year. period (1990 - 1995) with the exception of 1 year (1994) when a marked decline in antimicrobial utilization was noted. In the following years, amoxicillin and amoxicillin with enzyme inhibitor became the most-used antimicrobials. Conclusion: Various factors influenced antimicrobial utilization during the investigation period. There were factors that directly influenced prescribing, e.g. the physicians, prescribing habit, guidelines, policy and formulary. Other factors directly influenced utilization, e.g. availability of a drug through purchasing, dispensing, procurement, pricing etc.

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