Carbonylated Proteins ELISA Kit

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

sELISA

Intended Use

This ELISA Kit is intended for the determination of protein carbonyls in human serum and plasma.

Contents of Kit

1. PLATE Microtiter plate, pre-coated 12x 8 wells
2. WASHBUF Wash buffer concentrate, 10x 1x 100 ml
3. STD Standards, lyophilised
(see specification for concentrations) 4x 5 vials
4. CTRL 1 Control, lyophilised
(see specification for range) 4x 1 vial
5. CTRL 2 Control, lyophilised
(see specification for range) 4x 1 vial
6. CONJ Conjugate concentrate, peroxidase-labelled 1x 200 μl
7. CONJBUF Conjugate dilution buffer, ready-to-use 1x 15 ml
8. AB Detection antibody concentrate, (secondary antibody) 1x 200 μl
9. ABBUF Antibody dilution buffer, ready-to-use 1x 15 ml
10. DER Derivatisation reagent 2x 5ml
11. ASYBUF Assay buffer, ready-to-use 2x 100 ml
12. SUB Substrate (tetramethylbenzidine), ready-to-use 1x 15 ml
13. STOP Stop solution, ready-to-use 1x 15 ml

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date.

General Description

Reactive oxygen species (ROS) can oxidise proteins, lipids, and DNA, causing damage of their structure and function as well as cell injury. Proteins are oxidised by free radicals, whereby the constituent amino acids are variously modified or degraded. The modifications result in new functional groups such as carbonyl or hydroxyl groups, which may lead to protein fragmentation, formation of protein-protein cross-linkages, disruption of the tertiary structure and loss of functional activity. In addition, ROS are directly associated with diseases like atherosclerosis, rheumatoid arthritis, Alzheimer's and Parkinson's disease as well as ageing and cancerogenesis. Protein carbonyls are formed by a variety of oxidative mechanisms and are sensitive indices of oxidative injury. The quantity of protein carbonyls in a protein sample can be determined by derivatising with dinitrophenyl-hydrazine (DNPH) and measuring the bound anti-DNPH antibodies. The ELISA method enables carbonyls to be measured quantitatively with microgram quantities of protein.
Indications
• Atherosclerosis
• Alzheimer's disease
• Parkinson's disease
• Rheumatoid arthritis
• Uremia
• Diabetes
• Ageing
• Cancerogenesis