Carbaryl ELISA Kit (DEIANJ21)

Regulatory status: For research use only, not for use in diagnostic procedures.

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contaminated samples
Species Reactivity
Intended Use
The Carbaryl ELISA is an immunoassay for the detection of carbaryl in contaminated samples.
Store the kit at 2 - 8°C.
General Description
Carbaryl (1-naphthyl methylcarbamate) is a chemical in the carbamate family used chiefly as an insecticide. It is a cholinesterase inhibitor and is toxic to humans. It is classified as a likely human carcinogen by the United States Environmental Protection Agency (EPA.). The oral LD50 is 250 to 850 mg/kg for rats and 100 to 650 mg/kg for mice.[citation needed] A recent study reports that carbaryl is a structural mimic of the neurohormone melatonin and directly binds to MT2 melatonin receptor. This could significantly impact circadian rhythms and increase risk for diabetes and metabolic disorders.


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Sprayed liquid-gas extraction of semi-volatile organophosphate malathion from air and contaminated surfaces


Authors: Zarejousheghani, Mashaalah; Walte, Andreas; Borsdorf, Helko

In this study, a new air sampling method termed sprayed liquid-gas extraction (SLGE) was developed for semi-volatile organic compounds. Water droplets with an average diameter of less than 10 m were created, using a flow blurring nebulizer from distilled water and the gas-phase sample. This allowed the fast, simple and highly-efficient enrichment of trace levels of the widely used organophosphate insecticide malathion, which is also an accepted simulant for the potent nerve-agent VX. After spraying, extraction and solvent evaporation, the collected malathion molecules were dissolved in 100 L organic solvent and analyzed using gas chromatography-mass spectrometry. Different parameters including SLGE duration, gas and extracting-agent flow-rate, humidity and memory effect were evaluated and optimized. The method was compared with standard air-sampling tubes containing XAD-2 and XAD-4 polymers and also the liquid-trapping method. Combining simplicity with cost-effectiveness, the SLGE method shows outstanding extraction efficiency compared to the standard methods. Using the optimized method, the limits of detection (LOD) and quantification (LOQ) were determined to be 7.53 and 25.11 ng L-1, respectively. The developed method was compared to a standard extraction with XAD-2 tube which was run in parallel to the SLGE. These methods were used to extract a mixture of malathion, 2,4-dinitrotoluene (explosive marker), 4-chloro-3-methylphenol, carbaryl (insecticide), dimethoate (organophosphate insecticide), atrazine (herbicide) and permethrin (insecticide) in synthetic air. These compounds were also deposited on a steel plate and a composite building material and the air above these materials was sampled.

Study on Three-Dimensional Fluorescence Spectral Characteristics of Heterocyclic Pesticides in Different Environmental Conditions


Authors: Wu Wen-tao; Chen Yu-nan; Xiao Xue; Yang Rui-fang; Zhao Nan-jing

The impact analysis of different environments on the fluorescence emission spectrum of pesticides is critical in detecting the concentration of pesticides. In this paper, three kinds of pesticides, carbendazim, carbaryl and fuberidazole, were selected as the research objects. Under different environment, such as different pH values and the presence of different common anion or cation, three-dimensional fluorescence spectral emission (EEM) characteristic of pesticides were analyzed. The experimental results showed that the primary fluorescence peaks for three kinds of pesticides were at lambda(ex)/lambda(em) = 280/300, 310/340 and 280/335 nm (respectively); Carbendazim and fuberidazole had a secondary peak at 245/305 nm (PeakB) and 250/340 nm (PeakB). We can come to the conclusion that with the change of pH value, the characteristic of fluorescence emission of carbendazim and fuberidazole is similar. We can find that the fluorescence intensities of carbendazim and fuberidazole were enhanced with the declining of the solution acidity or alkalinity and the fluorescence intensity of carbaryl had not changed with the declining of the solution acidity, but it increased with the declining of the solution alkalinity; the fluorescence emission spectra of the three kinds of pesticides had good fluorescence characteristics with the scope of the pH varying from 6. 16 to 7. 4. Twelve common ions in water (CO32-, SO42-, NO3-, Cl-, HPO42-, HCO3-, Mg2+, Zn2+, NH4+, Na+, Ca2+, K+) had no significant effect on fluorescence emission characteristics of carbendazim and fuberidazole. The fluorescence intensities were seriously influenced by Fe3+ and Cu2+. The results showed that the pesticides fluorescence intensities were decreased with the ion concentration increasing. It was necessary to consider the quenching effects on pesticides of Fe3+ and Cu2+ for the analytic results. The obtained results provided the basic research for improving the accuracy of the heterocyclic pesticides measurement in water.

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