Carbamazepine ELISA Kit (DEIA6843)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Intended Use
The Carbamazepine ELISA Kit is an immunoassay for the quantitative and sensitive detection of Carbamazepine in water samples.
Contents of Kit
1. Microtiter plate
2. Standards (6)
3. Antibody solution, 6 mL
4. Carbamazepine-HRP, 6 mL
5. Diluent/zero, 25 mL
6. Wash Solution 5X concentrate, 100 mL
7. Color Solution (TMB), 12 mL
8. Stop Solution, 6 mL
Store all reagents at 4-8°C. Solutions should be allowed to reach room temperature (20-25°C) before use. For more detailed information, please download the following document on our website.
0.021 ppb


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The effect of cannabidiol on the pharmacokinetics of carbamazepine in rats


Authors: Darweesh, Ruba S.; Khamis, Tareq N.; El-Elimat, Tamam

Carbamazepine (CBZ) is mainly metabolized by CYP3A4 into carbamazepine-10,11-epoxide (CBZE). Cannabidiol (CBD) is a potent inhibitor of the CYP3A family. The aim of this study is to determine the effect of acute and chronic administration of CBD on the pharmacokinetics of CBZ and CBZE. Male SD rats were assigned into four acute and four chronic groups: control (CBZ only), positive control (ketoconazole), low-dose cannabidiol (l-CBD), and high-dose cannabidiol (h-CBD). Acute CBD groups were administered a single dose of CBD, while chronic CBD groups were given multiple doses of CBD for 14 days (q.d.) before CBZ administration. Plasma samples had been collected and analyzed for CBZ and CBZE, then their noncompartmental pharmacokinetic parameters before and after CBD administration were determined. The co-administration of a single l-CBD has significantly increased CBZ's AUC0 infinity{AUC}}_0<^>{\infty } $$\end{document} with 40.4% significant decrease in CBZE's C-max, when compared to the control. Chronic h-CBD caused a significant decrease in CBZ's C-max and AUC0 infinity}_0<^>{\infty } $$\end{document} by 75.3% and 65.7%, respectively. Besides, AUC0 infinity\ and C-max of CBZE significantly decreased by 75.3% and 78.3%, respectively. These results demonstrated that the pharmacokinetics of CBZ and CBZE had been significantly affected by CBD. When CBD has been administered as a single dose, the effect is believed to be mainly caused by the inhibition of CBZ metabolism through CYP3A. The effect of chronic administration of CBD probably includes kinetic pathways other than the inhibition of CYP3A-dependent pathways.

UVC-assisted photocatalytic degradation of carbamazepine by Nd-doped Sb2O3/TiO2 photocatalyst


Authors: Wang, Zhao; Srivastava, Varsha; Wang, Shaobin; Sun, Hongqi; Thangaraj, Senthil K.; Janis, Janne; Sillanpaa, Mika

The photocatalytic degradation of carbamazepine (CBZ) in ultra-pure water was investigated by using neodymium (Nd)-doped antimony trioxide (Sb2O3)/titanium dioxide (TiO2) photocatalyst under the UVC irradiations of 254 nm wavelength. The hydrothermal method was used for the fabrication catalyst samples with different ratios of Nd (0%-2%) dopant, and characterised by X-ray diffraction pattern (XRD) to investigate the crystallinity. Scanning electron microscopy (SEM) provided the surface morphologies, Bruanuer-Emmer-Teller (BET) analysis gave the textural properties, and UV-Vis diffuse reflectance absorption spectroscopy (DRS) was used for the investigation of the optical properties of synthesized catalysts. TEM images of Sb2O3 showed a nanorod-like structure while, in the Nd-doped Sb2O3/TiO2, a small dot-like structure was observed along with the nanorods. The surface area and band gap of 1% Nd-doped Sb2O3/TiO2 were found to be 9.56 m(2) g(-1) and 3.0 eV respectively. It was observed that the CBZ cannot be degraded in the absence of catalyst under UV light, while photocatalyst 1% Nd-doped Sb2O3/TiO2 at 0.5 g/L of catalyst dose showed the best photocatalytic activity towards CBZ degradation. The main degradation products were identified with high-resolution mass spectrometry. Moreover, the degradation of CBZ followed pseudo first-order kinetics and the rate constant was 0.017 min(-1). Quenching tests by the addition of methanol from 100 to 500 mM were carried out to determine the major reactive oxygen species, which showed that (OH)-O-center dot radicals was involved in the CBZ degradation. Active species-trapping experiments revealed that O-center dot(2)- is also responsible for the degradation of CBZ. (C) 2019 Elsevier Inc. All rights reserved.

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