Calprotectin (MRP 8/14) ELISA Kit (DEIA045J)

Regulatory status: For research use only, not for use in diagnostic procedures.

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serum, plasma
Species Reactivity
Intended Use
The described Calprotectin ELISA is intended for the quantitative determination of calprotectin (MRP (8/14, S100A8/A9)) in serum and plasma.
Contents of Kit
Unopened Kit: Store at 2-8°C. Do not use past kit expiration date.
Serum samples
Intra-Assay Precision: 4.48%-6.16%
Inter-Assay Precision: 7.49%-12.86%
Plasma samples
Intra-Assay Precision: 4.43%-5.28%
Inter-Assay Precision: 12.76-13.23%
3.2 ng/L


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Circular RNA circMAN2B2 facilitates glioma progression by regulating themiR-1205/S100A8 axis


Authors: Xiong, Jinsheng; Wang, Tianyao; Tang, Haitao; Lv, Zhonghua; Liang, Peng

The aim of this study was to research the mechanism of circMAN2B2 in the development of glioma. In our study, we found that circMAN2B2 has a higher expression in glioma tissues and cells, which was negatively related to the overall survival of glioma patients. The cell counting kit-8 assay, 5-ethynyl-2 '-deoxyuridine labeling assay, transwell assay, and the nude mice assay indicated that knockdown of circMAN2B2 inhibited the cell proliferation, invasion, migration and decreased tumor size. In terms of mechanism, knockdown of circMAN2B2 increased the expression of miR-1205. Moreover, circMAN2B2 regulated S100A8 expression by inhibiting miR-1205. We also showed that knockdown of S100A8 inhibited cell proliferation, invasion, and migration. Increasing S100A8 expression rescued the effect of si-circMAN2B2. In conclusion, circMAN2B2 could improve cell proliferation, invasion, and migration of the glioma by inhibiting miR-1205 and promoting the expression of S100A8.

Elemental Zn and its Binding Protein Zinc-alpha 2-Glycoprotein are Elevated in HPV-Positive Oropharyngeal Squamous Cell Carcinoma


Authors: Poropatich, Kate; Paunesku, Tatjana; Zander, Alia; Wray, Brian; Schipma, Matthew; Dalal, Prarthana; Agulnik, Mark; Chen, Si; Lai, Barry; Antipova, Olga; Maxey, Evan; Brown, Koshonna; Wanzer, Michael Beau; Gursel, Demirkan; Fan, Hanli; Rademaker, Alfred; Woloschak, Gayle E.; Mittal, Bharat B.

Human papillomavirus (HPV)-positive head and neck squamous cell carcinoma (HNSCC) is biologically distinct from HPV-negative HNSCC. Outside of HPV-status, few tumor-intrinsic variables have been identified that correlate to improved survival. As part of exploratory analysis into the trace elemental composition of oropharyngeal squamous cell carcinoma (OPSCC), we performed elemental quanitification by X-ray fluorescence microscopy (XFM) on a small cohort (n = 32) of patients with HPV-positive and -negative OPSCC and identified in HPV-positive cases increased zinc (Zn) concentrations in tumor tissue relative to normal tissue. Subsequent immunohistochemistry of six Zn-binding proteins-zinc-alpha 2-glycoprotein (AZGP1), Lipocalin-1, Albumin, S100A7, S100A8 and S100A9-revealed that only AZGP1 expression significantly correlated to HPV-status (p < 0.001) and was also increased in tumor relative to normal tissue from HPV-positive OPSCC tumor samples. AZGP1 protein expression in our cohort significantly correlated to a prolonged recurrence-free survival (p = 0.029), similar to HNSCC cases from the TCGA (n = 499), where highest AZGP1 mRNA levels correlated to improved overall survival (p = 0.023). By showing for the first time that HPV-positive OPSCC patients have increased intratumoral Zn levels and AZGP1 expression, we identify possible positive prognostic biomarkers in HNSCC as well as possible mechanisms of increased sensitivity to chemoradiation in HPV-positive OPSCC.

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