Calorimetry Service

Calorimetry Service

Isothermal Titration Calorimetry (ITC)

ITC is a powerful analytical tool which measures the binding affinity and thermodynamics between any two biomolecules. Binding constants in the range of 103 to 108 M-1 can be accurately measured.

ITC is considered the "gold standard" assay for binding, and has many advantages:

  • Universal assay - directly measures heat change associated with binding
  • Label-free - uses native materials
  • True in-solution technique
  • Requires minimal assay development
  • Has no molecular weight limitations
  • Non-optical
  • Versatile, can be used with any biomolecule - proteins, nucleic acids, small molecule drugs, lipids, etc. Can be used with a wide range of biological buffers, ionic strengths, pH's In a single ITC experiment, one can determine: 
  • Binding affinity - Kd
  • Directly measure sub-millimolar to nanomolar
  • Can extend affinity range to picomolar using competitive ITC method
  • Number of binding sites
  • Multiple and different binding sites
  • Enthalpy and entropy of binding

Differential Scanning Calorimetry (DSC)

DSC continuously measures heat capacity of a system as a function of temperature allowing for simultaneous determination of enthalpy, entropy and Gibbs energy for a thermal transition. This information has proven to be extremely important in the characterization of protein folding and oligonucleotide conformation. Differential Scanning Calorimetry (DSC) is a powerful analytical tool which directly measures the change in heat associated with the unfolding of a protein, lipid, or nucleic acid. In DSC, as the biomolecule is heated at a constant rate, a detectable heat change associated with thermal denaturation can be accurately measured. DSC experiments are:

  • Label-free and use native materials
  • True in-solution technique
  • Easy to perform-require minimal assay development
  • Conducted using a wide range of biological buffers, ionic strengths and pH's
  • Universal assay-measures the heat change associated with denaturation
  • Non-optical-unaffected by colored or turbid samples
  • Versatile-can be used with proteins, nucleic acids, lipids and other biomolecules

In a single DSC experiment, one can determine:

  • Transition midpoint (Tm)
  • Enthalpy and heat capacity change associated with unfolding

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