Sample
serum, cell culture supernatant
Intended Use
The CD's GRO/CINC-2 β EIA Kit is a complete kit for the quantitative determination of GRO/CINC-2 β in serum and supernatant of cell culture media.
Both recombinant and native forms of GRO/CINC-2 β can be detected with the kit
Contents of Kit
1 Precoated plate: Anti- Rat GRO/CINC-2 β Rabbit IgG Affinity Purify 96Well x 1
2 Labeled antibody: 10.5mL x 1
3 EIA buffer: 30mL x 1
4 Standard: 0.5mL x 1
5 Missing number
6 Stop solution: 11mL x 1
7 Chromogen: 15mL x 1
8 Wash buffer Conc.: 50mL x 1
9 Solution for Labeled antibody: 10.5mL x 1
Detection Range
9.4 - 600 pg/mL
General Description
GRO (Growth Related Oncogene) belonging to IL-8 family is polypeptide which has three isoforms a, β and γ, and it inhibits proliferation of endothelial cells. GRO/CINC-1 (cytokine-induced neutrophil chemo attractant 1) was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells (NRK-52E.) Amino acid sequence that encodes rat CINC-1 was identified in 1989 by Watanabe's group at Toyama Medical and Pharmaceutical University. CINC-1 is a member of the alpha (CXC) subfamily of chemokines. Three additional rat CXC chemokines (CINC-2a, CINC-2β, CINC-3/MIP-2) have been identified. The protein sequence of CINC-1 is 63 - 67% identical to that of CINC-2a, CINC-2β and CINC-3/MIP-2. In addition, each of GROa, GROβ and GRO? is sharing 68%, 71% and 69% identity with CINC-1. This has been suggested that CINCs are the rat counterparts of human GROs.
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