Cowpea mottle virus (CPSMV) ELISA Kit

Field- or greenhouse-grown plants are naturally exposed to microbes. Sequence data derived from these plants can therefore inadvertently include microbe sequences. We have examined the extent of viral sequences in public soybean (Glycine max and Glycine soja) sequence data. A collection of 303 149 soybean expressed sequence tags (ESTs) was computationally analyzed for sequences from 12 viruses infecting soybean: Alfalfa mosaic virus (AMV), Bean pod mottle virus (BPMV), Bean yellow mosaic virus (BYMV), Cowpea chlorotic mottle virus (CCMV), Cowpea mosaic virus (CPMV), Cowpea severe mosaic virus (CPSMV), Cucumber mosaic virus (CMV), Peanut mottle virus (PeMoV), Peanut stunt virus (PSV), Soybean mosaic virus (SMV), Tobacco ringspot virus (TRSV), and Tobacco streak virus (TSV). A total of 1652 sequences matching BPMV (1097 sequences), SMV (132 sequences), and CCMV (423 sequences) were discovered in the soybean EST collection. The viral sequences were assembled into contiguous sequences, and sequence tracts in common were used in a parsimony analysis of the phylogenetic relationship of putative viral genotypes. Our results include representative sequences from two BPMV subgroups and, surprisingly, many variants of BPMV nucleotide sequences. Furthermore, the results suggest that BPMV viral strains may be mixes of different BPMV RNA-1 and RNA-2 genotypes. This study presents the largest collection of sequence data available to date for the BPMV, SMV, and CCMV viruses and represents the first report of this magnitude of varieties of BPMV nucleotide sequences.

Cowpea severe mosaic virus (CPSMV) causes significant losses in cowpea (Vigna unguiculata) production. In this present study biochemical, physiological, and proteomic analysis were done to identify pathways and defense proteins that are altered during the incompatible interaction between the cowpea genotype BRS-Marataoa and CPSMV. The leaf protein extracts from mock- (MI) and CPSMV-inoculated plantlets (V) were evaluated at 2 and 6 days post-inoculation (DPI). Data support the assumptions that increases in biochemical (high hydrogen peroxide, antioxidant enzymes, and secondary compounds) and physiological responses (high photosynthesis index and chlorophyll content), confirmed by label-free comparative proteomic approach, in which quantitative changes in proteasome proteins, proteins related to photosynthesis, redox homeostasis, regulation factors/RNA processing proteins were observed may be implicated in the resistance of BRS-Marataoa to CPSMV. This pioneering study provides information for the selection of specific pathways and proteins, altered in this incompatible relationship, which could be chosen as targets for detailed studies to advance our understanding of the molecular, physiological, and biochemistry basis of the resistance mechanism of cowpea and design approachs to engineer plants that are more productive. Biological significance: This is a pioneering study in which an incompatible relationship between a resistant cowpea and Cowpea severe mosaic virus (CPSMV) was conducted to comparatively evaluate proteomic profiles by Gel-free/label-free methodology and some physiological and biochemical parameters to shed light on how a resistant cowpea cultivar deals with the virus attack. Specific proteins and associated pathways were altered in the cowpea plants challenged with CPSMV and will contribute to our knowledge on the biological process tailored by cowpea in response to CPSMV. (C) 2017 Elsevier B.V. All rights reserved.