Mouse COL3(Collagen Type III) ELISA Kit

The electrochemical oxidation of anodic cobalt in an acetonitrile solution of Schiff bases (HL) derived from H-pyrrole-2-carbaldehyde and substituted anilines gives solutions from which [CoL3] complexes were obtained. When 1,10-phenanthroline (phen) or 2,2'-bipyridine (bipy) was added to the electrolytic phase, [CoL2 phen] or [CoL2 bipy] are obtained. The crystal structure of tris{2-[(2-methylphenyl)iminomethyl]pyrrolato} cobalt(III) has been determined by X-ray diffraction. The crystal structure consists of monomeric molecules in which the central CoN6 unit has a slightly distorted octahedral geometry. The electronic, IR and H-1 NMR spectra of the complexes are discussed and related to the structure.

Dermal fibroblasts/myofibroblasts involved in the wound healing are thought to originate from the resident fibroblast progenitors. To test the hypothesis of an extra dermal origin of the dermal fibroblasts/myofibroblasts, bone marrow (BM) transplantation and parabiosis experiments were initiated utilizing a collagen promoter green fluorescent protein (GFP) reporter transgene as a visible marker for dermal fibroblasts/myofibroblasts. BM transplantation experiments using BM from Co13.6GFPsapph transgenic mice showed no evidence that BM derived progenitors differentiated into dermal fibroblasts/myofibroblasts at the wound site. Rather the GFP positive cells (GFP+) observed at the wound site were not dermal fibroblasts/myofibroblasts but immune cells. These GFP+ cells were also detected in the lung and spleen. Furthermore, GFP+ fibroblasts were not detected in primary dermal fibroblast cultures initiated from BM chimeras. Using the same transgenic mice, parabiotic pairs were generated. One partner in the parabiosis carried a GFP expressing transgene while the other partner was a non-transgenic C57BL/6 mouse. Similar to the BM transplantation experiments, GFP+ immune cells were detected in the wound of the non-transgenic parabiont, however, GFP expressing dermal fibroblasts/myofibroblasts were not observed. Collectively, these data suggest that dermal fibroblast/myofibroblast progenitors do not readily circulate. The expression of the Col3.6GFPsapph in the hematopoietic cells confirmed that our methods were sensitive enough to detect Col3.6GFP expressing dermal fibroblasts derived from the peripheral circulation if they had originated in the BM. J. Cell. Physiol. 222: 703-712, 2010. (C) 2009 Wiley-Liss, Inc.