plasma, serum, urine, saliva, milk, CSF, cell culture samples
The CD Human Complement Factor H ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of CFH in human plasma, serum, urine, saliva, milk, CSF, and cell culture samples.
- Upon arrival, immediately store components of the kit at recommended temperatures up to the expiration date.
- Store SP Conjugate and Biotinylated Antibody at -20°C.
- Store Microplate, Diluent Concentrate (10x), Wash Buffer, Stop Solution, and Chromogen Substrate at 2-8°C.
- Unused microplate wells may be returned to the foil pouch with the desiccant packs and resealed. May be stored for up to 30 days in a vacuum desiccator.
- Diluent (1x) may be stored for up to 30 days at 2-8°C.
- Store Standard at 2-8°C before reconstituting with Diluent and at -20°C after reconstituting with Diluent.
The minimum detectable dose of human CFH as calculated by 2SD from the mean of a zero standard was established to be 0.25 ng/ml.
Complement factor H (CFH, H factor 1) is a 1213-residue plasma glycoprotein that regulates the function of the alternative complement pathway. The CFH gene encodes a 155-kDa protein containing 20 tandem complement control protein (CCP) modules, also known as short consensus repeats (about 60 amino acids each), and an alternative spliced 45-kDa protein. It binds to C3b to accelerate the decay of the C3 convertase C3bBb and also acts as a cofactor for complement factor I-mediated C3b cleavage. Human CFH is particularly important for selectively protecting self-surfaces by binding to glycosaminoglycans on host cells.