CDK5 (Phospho-Tyr15) ELISA Kit (DEIA-XYA389)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
2 x 96T
Sample
cultured cells
Species Reactivity
Human, Mouse, Rat
Intended Use
The CDK5 (Phospho-Tyr15) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CDK5 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated CDK5 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CDK5 phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL (10x)
3. Quenching Buffer: 24 mL (1x)
4. Blocking Buffer: 50 mL (1x)
5. 10x Wash Buffer: 50 mL (10x)
6. 100x Anti-CDK5 (Phospho-Tyr15) Antibody (Rabbit Polyclonal): 60 μL (100x), Red
7. 100x Anti-CDK5 Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x), Glass
11. Primary Antibody Diluent: 12 mL (1x)
12. Ready-to-Use Substrate: 12 mL (1x), Brown
13. Stop Solution: 12 mL (1x)
14. Crystal Violet Solution: 12 mL (1x), Glass
15. SDS Solution: 24 mL (1x)
16. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months

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References


Nakamura, S; Kawamoto, Y; et al. Cyclin-dependent kinase 5 and mitogen-activated protein kinase in glial cytoplasmic inclusions in multiple system atrophy. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY 57:690-698(1998).
Augustinack, JC; Sanders, JL; et al. Colocalization and fluorescence resonance energy transfer between cdk5 and AT8 suggests a close association in pre-neurofibrillary tangles and neurofibrillary tangles. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY 61:557-564(2002).

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