Calnexin (Phospho-Ser583) ELISA Kit (DEIA-XYA326)

Regulatory status: For research use only, not for use in diagnostic procedures.

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2 x 96T
cultured cells
Species Reactivity
Human, Mouse, Rat
Intended Use
The Calnexin (Phospho-Ser583) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Calnexin protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Calnexin in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Calnexin phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL (10x)
3. Quenching Buffer: 24 mL (1x)
4. Blocking Buffer: 50 mL (1x)
5. 10x Wash Buffer: 50 mL (10x)
6. 100x Anti-Calnexin (Phospho-Ser583) Antibody (Rabbit Polyclonal): 60 μL (100x), Red
7. 100x Anti-Calnexin Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x), Glass
11. Primary Antibody Diluent: 12 mL (1x)
12. Ready-to-Use Substrate: 12 mL (1x), Brown
13. Stop Solution: 12 mL (1x)
14. Crystal Violet Solution: 12 mL (1x), Glass
15. SDS Solution: 24 mL (1x)
16. Adhesive Plate Seals: 4 seals
4°C/6 Months


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Design and Operation of the GPS Receiver Onboard the CanX-2 Nanosatellite


Authors: Kahr, Erin; O'Keefe, Kyle P. G.; Skone, Susan; Bradbury, Laura

Among its payloads, the Canadian Advanced Nanospace eXperiment 2 (CanX-2) nanosatellite is carrying a commercial off-the-shelf GPS receiver, which offers advantages in terms of cost, size and power, and overall continues to perform well more than four years after launch. However, at orbital velocity and with low signal power the receiver is unable to acquire a position fix quickly using its standard acquisition algorithm. In order to effectively collect GPS data over short time intervals, the receiver is manually warm started by pre-assigning channels with PRN and Doppler information. Uploading warm start scripts to the satellite allows for reliable acquisition in an average of 3.5 min compared to a cold start of approximately 20 min, and avoids the need for complex onboard warm start capability. The data collection capability has in turn enabled a variety of scientific results to be obtained. Copyright (C) 2013 Institute of Navigation.

Depletion of endogenous miRNA-378-3p increases peak cell density of CHO DP12 cells and is correlated with elevated levels of ubiquitin carboxyl-terminal hydrolase 14


Authors: Costello, Alan; Coleman, Orla; Lao, Nga T.; Henry, Michael; Meleady, Paula; Barron, Niall; Clynes, Martin

miRNAs are potent molecular regulators of cellular behaviour. The manipulation of these small non-coding RNAs has been used to enhance industrially relevant phenotypes in Chinese Hamster Ovary (CHO) cells. We investigated the stable depletion of six miRNAs; miR-204-5p, 338-3p, 378-3p, 409-3p, 455-3p and 505-3p, robustly associated with cell growth rate from a previous profiling study. Inhibition of endogenous miR-378-3p function by miRNA-sponge-decoy improved peak cell density by 59%. Quantitative label free LC-MS/MS proteomic analysis of the fractionated cell cultures at day 4 and 8 of batch culture found 216 cytosolic and 114 membrane-associated proteins differentially expressed with stable miR-378-3p depletion. qRT-PCR of 8 genes; Clic4, Hnrnpa1, Prdx1, Actn4, Usp14, Srxn1, Canx and Gnb1, with unidirectional differential protein expression over the two time points of analysis was carried out. In-silico predictive algorithms; TargetScan and miRDB, were used to decipher possible direct targets of miR-378-3p. The Ubiquitin carboxyl-terminal hydrolase 14 (Usp14) protein was identified in the cytosolic fractions at both timepoints as differentially expressed with an increased abundance of 1.58-fold in the miR-378-3p depleted cells on day 8. Usp14 is a deubiquitinase (DUB) with previous reports of its up-regulation leading to increased proliferation of cancer cells. Overexpression of Usp14 in CHO cells had significant effects on cell growth supporting a role for Usp14 in the increased peak cell density seen with miR-378-3p depletion. This study highlights miR-378-3p as a novel engineering candidate for improving CHO cell growth. The use of subcellular fractionation also improved proteome coverage in the identification of novel miRNA targets.

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