Rabbit Anti-Human DFFB (internal) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Synthetic peptide corresponding to a region derived from internal residues of human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)

Conjugate

Application Notes

IHC: 1:25-1:100

General Notes

This product (Catalog No. CABT-BL826) is a rabbit-derived IgG polyclonal antibody raised against human DFFB. It has been affinity purified by protein A. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.
CABT-BL826 is suitable for use in IHC (1:25–1:100) application.
CABT-BL826 is supplied as a liquid formulation in pH7.4 PBS, 0.05% sodium azide, 40% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

CAD; CPAN; DFF2; DFF40; DFF-40

Entrez Gene ID

UniProt ID