CA-242(Pancreatic-colon-rectal) ELISA Kit (DEIA1999)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum
Species Reactivity
Human
Intended Use
The CA-242 ELISA is a solid phase enzyme linked immunosorbent assay (ELISA). This test provides measurement of CA-242 antigen.
Contents of Kit
1. Microwell Strips(96 wells)
2. Biotinylated Capture Antibody Solution
3. Enzyme Conjugate
4. Specimen Diluent
5. Reference Standards
6. Low and High Control
7. TMB Solution
8. Washing Buffer Concentrate
9. Stop Solution
Storage
This test kit must be stored at 2-8°C upon receipt. For more detailed information, please download the following document on our website.
Detection Range
5-200 U/mL
Detection Limit
5 U/mL
Sensitivity
5 U/mL
Standard Curve

Citations


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CA242 ELISA Kit (DEIA06037)

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References


THE CALCIUM-ACTIVATED POTASSIUM CHANNELS OF TURTLE HAIR-CELLS

JOURNAL OF GENERAL PHYSIOLOGY

Authors: ART, JJ; WU, YC; FETTIPLACE, R

A major factor determining the electrical resonant frequency of turtle cochlear hair cells is the time course of the Ca-activated K current (Art, J. J., and R. Fettiplace. 1987. Journal of Physiology. 385:207-242). We have examined the notion that this time course is dictated by the K channel kinetics by recording single Ca-activated K channels in inside-out patches from isolated cells. A hair cell's resonant frequency was estimated from its known correlation with the dimensions of the hair bundle. All cells possess BK channels with a similar unit conductance of similar to 320 pS but with different mean open times of 0.25-12 ms. The time constant of relaxation of the average single-channel current at -50 mV in 4 mu M Ca varied between cells from 0.4 to 13 ms and was correlated with the hair bundle height. The magnitude and voltage dependence of the time constant agree with the expected behavior of the macroscopic K(Ca) current, whose speed may thus be limited by the channel kinetics. All BK channels had similar sensitivities to Ca which produced half-maximal activation for a concentration of similar to 2 mu M at +50 mV and 12 mu M at -50 mV. We estimate from the voltage dependence of the whole-cell K(Ca) current that the BK channels may be fully activated at -35 mV by a rise in intracellular Ca to 50 mu M. BK channels were occasionally observed to switch between slow and fast gating modes which raises the possibility that the range of kinetics of BK channels observed in different hair cells reflects a common channel protein whose kinetics are regulated by an unidentified intracellular factor. Membrane patches also contained 30 pS SK channels which were similar to 5 times more Ca-sensitive than BK channels at -50 mV. The SK channels may underlie the inhibitory synaptic potential produced in hair cells by efferent stimulation.

Evaporation residue cross-section in the decay of No-254* formed in Pb-206+Ca-48 and its isotopic dependence using other Pb targets within the dynamical cluster-decay model

NUCLEAR PHYSICS A

Authors: Niyti; Gupta, Raj K.; Hess, Peter Otto

The dynamical cluster-decay model (DCM), with deformation and orientation effects included, is used to calculate the fusion evaporation residue cross-sections sigma(xn) for x =1,2, 3 and 4 neutrons emission in a fusion reaction Pb-206 + Ca-48 -> No-254* at various Ca-48-beam energies E-lab = 212.7-242.5 MeV (equivalently, E* = 19.8-43.9 MeV). Considering the higher multipole deformations up to hexadecapole deformation beta(4i) and the sticking moment-of-inertia I-S, the DCM with pocket formula for nuclear proximity potential is shown to give a good description of the measured individual light-particle (here neutrons) decay channels for configurations of "hot, compact" orientations theta(ci), within one parameter fitting of the neck-length Delta R. A check on some of the variables involved in DCM shows that (i) spherical configurations give nearly the same result as above for deformed and oriented ones; (ii) the non-sticking moment-of-inertia I-NS gives unphysical results; and (iii) configurations of "cold, elongated" orientations do not fit the data at all. Furthermore, for the four different isotopes of Pb-204,Pb-206,Pb-207,Pb-208-based reactions, the dependence of, say, the 2n-emission yield sigma(2n) on the isotopic composition of the compound nucleus is also studied within the DCM for "hot" fusion process. Of all the four Pb-isotopes and three excitation energies E* considered, at each E*, the Delta R is largest for compound system No-256*, followed by No-255*, No-254* and smallest for No-252*, which means to suggest that the neutrons emission occur earliest for No-256*, then for No-255*, No-254* and finally by No-252*, in complete agreement with experimental data according to which compound system No-256* has the highest cross-section and No-252* the lowest with No-255* and No-254* lying in between. This result is related to the double magicity of both the target (Pb-208) and projectile (Ca-48) nuclei, as well as to the experimentally known result of projectile with a larger number of neutrons (here the target nucleus Pb) showing an increase in the production cross-section of superheavy nuclei, and is shown to be mainly due to the penetrability factor P in the compound nucleus decay cross-section. (C) 2015 Elsevier B.V. All rights reserved.

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