Human Raf1 blocking peptide (CDBP0876)

Synthetic Human Raf1 blocking peptide for BL

Product Overview
Blocking peptide for anti-C-raf antibody
Target
C-raf
Nature
Synthetic
Species Reactivity
Human
Tag/Conjugate
Unconjugated
Application Notes
For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not suitable for human or animal consumption.
Procedure
None
Format
Liquid
Concentration
200 μg/ml
Size
50 μg
Buffer
PBS containing 0.02% sodium azide
Preservative
0.02% Sodium Azide
Storage
Store at -20℃, stable for one year.
UniProt ID
Antigen Description
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Function
ATP binding; MAP kinase kinase kinase activity; Ras GTPase binding; metal ion binding; mitogen-activated protein kinase kinase binding; nucleotide binding; protein binding; protein heterodimerization activity; protein kinase activity; protein serine/threo
Synonyms
RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; RAF proto-oncogene serine/threonine-protein kinase; c Raf; CRAF; Raf 1; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; NS5; Raf-1; c-Raf;

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References


Transgenic overexpression of rubisco subunits and the assembly factor RAF1 are beneficial to recovery from drought stress in maize

ENVIRONMENTAL AND EXPERIMENTAL BOTANY

Authors: Doron, Lior; Xu, Lixin; Rachmilevitch, Shimon; Stern, David B.

Finding ways to increase Rubisco activity in crops to improve photosynthesis has long been a key objective. We have developed maize plants with transgenic overexpression of Rubisco subunits and Rubisco assembly factor 1 (RAF1) that resulted in higher Rubisco content, photosynthetic capacity and dry biomass under non-stressed growth conditions. The question remains, however, whether the transgenes conferring these effects could confer a growth penalty under stress conditions, given the nitrogen sink that Rubisco represents. In the present study, control and transgenic maize lines either overexpressing RAF1, both large and small subunits of Rubisco (LSSS), or all three (RAF1-LSSS), were subjected to mild, and severe drought conditions, and then re-watered. The results demonstrate that RAF1-LSSS, which has increased Rubisco abundance relative to the control, was slightly more affected than wild-type Rubisco level plants in terms of gas exchange and chlorophyll content after 14 days of stress. After returning plants to well-watered conditions, however, RAF1-LSSS displayed a more robust recovery compared to other genotypes. We attribute this to its high Rubisco content and the low malondialdehyde content noted prior to recovery, which indicates reduced oxidative degradation of lipids. Strong recovery was also observed in lines overexpressing RAF1 alone, in contrast with its lack of improved performance under non-stressed conditions. In contrast, LSSS displayed improved growth under non-stressed conditions, but showed a clear growth penalty during stress along with poor recovery. Taken together, our results suggest that while the high-Rubisco trait is neutral during water stress, recovery is more rapid once the stress is removed.

RAF1 variant in a patient with Noonan syndrome with multiple lentigines and craniosynostosis

AMERICAN JOURNAL OF MEDICAL GENETICS PART A

Authors: Rodriguez, Fernando; Ponce, Diana; Berward, Francisco J.; Lopetegui, Bernardita; Cassorla, Fernando; Aracena, Mariana

We report the case of a 14 years and 8 months girl, who is the first child of nonconsanguineous parents, with short stature, obstructive hypertrophic cardiomyopathy, multiple facial lentigines, high and wide forehead, downslanting palpebral fissures, low-set ears, short neck, and pectus excavatum; all features suggestive of Noonan syndrome with multiple lentigines (NSML). In addition, the patient exhibited craniosynostosis. Molecular analysis of rats sarcoma (RAS)/mitogen-activated protein kinase (MAPK) pathway genes with high-resolution melting curve analysis followed by sequencing showed a RAF1 amino acid substitution of valine to glycine at position 263 (p.V263G). The present report provides clinical data regarding the first association of a RAF1 variant and craniosynostosis in a patient with clinical diagnosis of NSML.

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