Magic™ Anti-C. difficile Toxin A polyclonal antibody (CABT-B1084)

Goat Anti-C. difficile Toxin A polyclonal antibody for ELISA, sELISA

Specifications


Host Species
Goat
Species Reactivity
C. difficile
Immunogen
C. diffieile Toxin A
Conjugate
Unconjugated

Applications


Application Notes
Direct ELISA: The antibody titer,measured at the midpoint was 1:20000 using a 2 μg coating of C. difficile Toxin A.
We recommend the following for sandwich ELISA (Capture - Detection):
CABT-B1970 - CABT-B1084
For example, a 1 μg coating of CABT-B1970 fllowed by exposure to the antigen and a 1:1000 dilution of the goating antisera gives a midpoint of approximately 40 ng/ml. As low as 1 ng/ml of antigen is readily detected using a standard chromogenic substrate.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
tcdA; toxA; Clostridium difficile Toxin A

Citations


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We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


CIESIELSKITRESKA, J; ULRICH, G; et al. PHOSPHORYLATION OF CELLULAR PROTEINS IN RESPONSE TO TREATMENT WITH CLOSTRIDIUM-DIFFICILE TOXIN-B AND CLOSTRIDIUM-SORDELLII TOXIN-L. EUROPEAN JOURNAL OF CELL BIOLOGY 56:68-78(1991).
Nguyen, BP; Gil, SG; et al. Deposition of laminin 5 by keratinocytes regulates integrin adhesion and signaling. JOURNAL OF BIOLOGICAL CHEMISTRY 275:31896-31907(2000).

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