Magic™ Anti-C. difficile Binary Toxin B Subunit polyclonal antibody (CABT-B1085)

Chicken Anti-C. difficile Binary Toxin B Subunit polyclonal antibody for sELISA

Specifications


Host Species
Chicken
Antibody Isotype
IgY
Species Reactivity
C. difficile
Immunogen
C. difficile Binary Toxin, B Subunit
Conjugate
Biotin

Applications


Application Notes
We recommend the following for sandwich ELISA (Capture - Detection):
CABT-B1969 - CABT-B1085
A 1 μg coating of CABT-B1969 is used as the capture antibody followed by exposure to the antigen. The antigen is detected using a 1:1000-1:5000 dilution of CABT-B1085 with a Peroxidase conjugated Neutravidin and a fluorogenic substrate. The midpoint of the binding curve is approximately 70 ng/ml. As low as 1 ng/ml of antigen is readily detected.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
C. difficile, B Subunit Binary Toxin

Citations


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Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
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References


Steele, J; Chen, K; et al. Systemic Dissemination of Clostridium difficile Toxins A and B Is Associated With Severe, Fatal Disease in Animal Models. JOURNAL OF INFECTIOUS DISEASES 205:384-391(2012).
Na, X; Zhao, DZ; et al. Clostridium difficile toxin B activates the EGF receptor and the ERK/MAP kinase pathway in human colonocytes. GASTROENTEROLOGY 128:1002-1011(2005).

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