Sample
serum, plasma or cerebrospinal fluid
Intended Use
The ELISA Borrelia burgdorferi IgM is quantitative and qualitative test for detection of human antibodies in serum, plasma or cerebrospinal fluid directed against Borrelia burgdorferi sensu lato. For sale in the U.S. for Research Use Only. Not for use in diagnostic procedures.
Contents of Kit
1. Break apart microtiter test strips each with 8 antigen coated single wells (CV 96), 1 frame, the coating material is inactivated. 12×8
2. Standard serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV-Ab; preservative: < 0.1 % sodium azide colouring: Amaranth O. 2 × 2 ml
3. Negative control serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV-Ab; preservative: < 0.1 % sodium azide colouring: Lissamine green V
4. Anti-human-IgM-conjugate (ready-to-use), Anti-human-IgM from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane
5. Washing solution concentrate (sufficient for 1000 ml), Sodium chloride solution with Tween 20, 30 mM Tris preservative: < 0.1 % sodium azide. 33.3 ml
6. Dilution buffer S2, (Special dilution buffer only for ELISA Borrelia burgdorferi IgG. Shake immediately before use!) Phosphate buffer with protein and Tween 20; ultrasonicate of Treponema, phagedenis preservative: < 0.1 % sodium azide 0.01 g/l Bromphenol blue sodium salt
7. Stopping solution, 1.2 N sodium hydroxide, 15 ml.
8. Substrate (ready-to-use), Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide, (Substrate in unopened bottle may have a slightly yellow color. This does not reduce the quality of the product!)
Storage
1. microtiter strips (antigen). Unopened: see expiry date on microtiter plate. after opening at 2-8°C in closed aluminum bag with desiccant: minimum shelf-life 4 weeks. Strips which are not used must be stored in the press-seal bag of aluminum compound foil under dry and airtight conditions!
2. control sera / standard sera. after opening at 2-8°C. until expiry date; 24 months from date of production
3. conjugate. ready-to-use solution at 2-8°C. Avoid contamination (sterile tips!). Until expiry date,
28 months from date of production.
4. dilution buffer. After opening at 2-8°C (Discard cloudy solutions!), 24 months. Unopened, until expiry date; 36 months from date of production.
5. washing solution. concentrate after opening at 2-8°C: until expiry date. Working dilution at 2-8°C: 2 weeks. Working dilution at room temperature: 1 week. Bottles used for the working dilution should be cleaned regularly. Discard cloudy solutions.
6. substrate. ready-to-use solution at 2-8°C, protected from light! until expiry date, 24 months from date of production. Avoid contamination (sterile tips!) Discard when solution turns yellow (extinction against distilled water > 0.25).
7. stopping solution. after opening at room temperature. until expiry date
General Description
Borrelia burgdorferi is the infectious agent which causes the disease syndrome known as Lyme-Borreliosis, named after the town Old Lyme in Connecticut (USA). The infectious agent was discovered in 1982 by Willy Burgdorfer and is classified into the taxonomic group of the spirochetes. The bacteria are transmitted to human hosts by infected ticks (Ixodes ricinus, Europe; Ixodes scapularis, USA). B. burgdorferi sensu stricto, B. garinii and B. afzelii are the most important human pathogens of the genospecies Borrelia burgdorferi sensu lato. All three are distributed throughout Europe in all temperate climate zones while in the USA human pathogenic strains belong to the species B. burgdorferi sensu stricto. Reservoirs for the bacteria in the wild include a variety of small wild mammals, particularly mice. The infection rate of adult ticks, nymphs and larvae with Borrelia burgdorferi ranges from 0-50%, dependent upon geographical region and tick populations, while in endemic regions of Germany the infection rate is 10-30 %.
Lyme Disease is a multisystemic infection with many possible manifestations. Single or multiple organs may be involved. The time course of Lyme Disease can be divided into three separate stages: Stage I, early localized infection, Stage II, early disseminated infection, and Stage III, late persistent infection.
A European strain of the genospecies Borrelia garinii in addition to the strain PKo has been used as antigen for coating on microtiter wells. The genospecies used expresses the proteins OspC and VlsE and P100. To increase the test´s sensitivity, recombinant VlsE is added to the coating antigen. Specificity of the assay is optimized by supplementing the dilution buffer with a treponema phagedenis lysate. The lysate absorbs potentially crossreactive treponema antibodies.
Citations
Publication ()
Have you cited DEIA1975 in a publication?
Let us know and earn a reward for your research.
COA
![The purity of Recombinant B.burgdorferi OspC protein [His] >95%, determined by SDS-PAGE](https://resource.creative-diagnostics.com/diagnostics/productimages-1/DAGA-2006-1.jpg)
Loading ......
