Bordetella FHA IgG ELISA kit (DEIA-XY70)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Serum, plasma
Species Reactivity
N/A
Intended Use
The Bordetella FHA IgG Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgG antibodies against Bordetella FHA in serum and plasma.
Storage
Store kit components at 2-8°C. After use, the plate should be resealed, the bottle caps replaced and tightened and the kit stored at 2-8°C. The opened kit should be used within three months.
Precision
Intra-Assay-Precision: 4.8 – 7.2 %
Inter-Assay-Precision: 6.2 – 17.0 %
Inter-Lot-Precision: 2.6 - 23.1 %
Detection Range
25 – 250 IU/mL
Sensitivity
0.5 IU/mL
General Description
Whooping cough is a disease of the respiratory tracts which is caused by Bordetella pertussis bacteria. It is transmitted by airborne infection. The gramnegative Coccobacillus produces a series of biologically active molecules. The different compounds appear either during the pathogenesis or during the process of immunization against pertussis and show different effects. A characterisation has been made for the pertussis toxin (pt), the filamentery haemagglutinine (fha) and different lipopolysaccharides (lps).
Pertussis shows a high rate of transmission (rates of infection of over 90 % have been found for non-vaccinated household members) and can cause severe diseases, especially for very young children. In older children and adults the disease may manifests itself by unspecific brochitic symptoms or inflammation of the upper respiratory tract. School children which are not totally immune may develop whooping cough and infect younger children easily. This applies also to infected adults. The development of antibodies towards Bordetella pertussis during a pertussis disease reveals itself by a relatively long serologically negative initial phase, followed by a rapid increase of at first IgA und IgM antibodies and lastly IgG. Of 259 sera derived from children and adults with suspected whooping cough 142 were confirmed positive by an ELISA. Due to the broad application of Bordetella vaccines a significant reduction of the incidence and fatal casualties caused by whooping cough were achieved. A precise method for the quantification of the antibody concentration against Bordetella pertussis in serum is required to assess the success of a pertussis vaccination, diagnose an infection and detect diaplancental transfer of maternal antibodies.
FHA is a 220 kDa sized surface antigen of Bordetella pertussis and Bordetella parapertussis which agglutinates erythrocytes in vitro. It is a component of numerous vaccines. For this reason a simple, fast, sensitive and according to an international reference preparation calibrated ELISA test kit for the detection of Bordetella FHA IgG antibodies was developed.

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References


Kindrachuk, J; Jenssen, H; et al. A novel vaccine adjuvant comprised of a synthetic innate defence regulator peptide and CpG oligonucleotide links innate and adaptive immunity. VACCINE 27:4662-4671(2009).
Donato, GM; Goldsmith, CS; et al. Delivery of Bordetella pertussis adenylate cyclase toxin to target cells via outer membrane vesicles. FEBS LETTERS 586:459-465(2012).

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