Biotin ELISA Kit (DEIA9990)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, foods
Species Reactivity
N/A
Intended Use
The Biotin ELISA Kit is intended for the quantitative determination of Biotin in serum, plasma and foods.
Contents of Kit
1. One holder with precoated strips, 96
2. ELISA wash buffer concentrate 10 x, 2 x 25 mL
3. Sample dilution buffer, ready-to-use, 1 x 15 mL
4. Conjugate dilution buffer, ready-to-use, 1 x 25 mL
5. Calibrators, ready-to-use (red cap)(0; 37.5; 75; 150; 300; 600 ng/l), 6 x 1 mL
6. Conjugate, horseradish peroxidase labeled streptavidin (green cap), 1 x 0.3 mL
7. Controls, ready-to-use (yellow cap), 2 x 1 mL
8. TMB Substrate (Tetramethylbenzidin), ready-to-use, 1 x 12 mL
9. ELISA Stop solution, ready-to-use, 1 x 12 mL
Storage
For more detailed information, please download the following document on our website.

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References


Multiple Progressive Thermopreconditioning Improves Cardiac Ischemia/Reperfusion-induced Left Ventricular Contractile Dysfunction and Structural Abnormality in Rat

TRANSPLANTATION

Authors: Chen, Yueh-Hsi; Chiang, Chih-Yao; Chang, Tzu-Ching; Chien, Chiang-Ting

Background. Triple progressive thermopreconditioning (3PTP) may induce high Hsp-70 expression to maintain cardiac function. We suggest that 3PTP may reduce myocardial ischemia/reperfusion (I/R) injury during organ transplantation through Bag3/Hsp-70 mediated defense mechanisms. Methods. Male Wistar rats were divided into sham control group and 72 h after 3PTP in a 42 degrees C water bath (3PTP) group. Rats underwent 60 min of ischemia by occlusion of the left anterior descending coronary artery followed by 240 min reperfusion. Hemodynamic parameters, including the electrocardiogram, microcirculation, heart rate, left ventricular end-diastolic pressure, maximal rate of rise (+dp/dt), and fall (-dp/dt) in the left ventricular pressure for index of contraction and relaxation were determined. Myocardial infarct size was evaluated by the Evans blue-2,3,5-triphenyltetrazolium chloride method. 3PTP-induced protective mechanisms were determined by Western blot and immunohistochemistry. Results. Cardiac I/R depressed cardiac microcirculation, induced S-T segment elevation, and R-R and P-R interval elongation increased infarct size associated with erythrocyte extravasation, leukocytes and macrophage/monocyte infiltration, granulocyte colony-stimulating factor, poly(ADP-ribose) polymerase 1 stain, and transferase-mediated dUTP-biotin nick end labeling positive cells. However, 3PTP evoked significant cardioprotection against I/R injury, characterized by the increased +dp/dt value and the decreased elevated left ventricular end-diastolic pressure, erythrocyte extravasation, leukocyte and macrophage/monocyte infiltration, granulocyte colony-stimulating factor expression, poly(ADP-ribose) polymerase 1 expression, transferase-mediated dUTP-biotin nick end labeling positive cells, and fragmentation and infarct area. In addition, 3PTP increased Hsp-70 and Bag3 expression and decreased Bax/Bcl-2 ratio, but did not affect the Beclin-1 and LC3-II/LC3-I ratio in the heart with I/R injury. Conclusions. 3PTP therapies may through Bag3 upregulation alleviate I/R injury-induced left ventricular structural deterioration and dysfunction.

Development of a novel polyprobe for simultaneous detection of six viruses infecting stone and pome fruits

3 BIOTECH

Authors: Noorani, Md Salik; Khan, Jawaid Ahmad

A biotin-labeled, non-isotopic, novel polyprobe was developed for the simultaneous detection of six viruses viz. apple chlorotic leaf spot virus (ACLSV), apple mosaic virus (ApMV), apple stem grooving virus (ASGV), cherry virus A (CVA), prunus necrotic ringspot virus (PNRSV) and plum pox virus (PPV) infecting stone and pome fruit trees through dot-blot hybridization assay. The sensitivity of the polyprobe was checked by serial dilutions of total RNA extracted from the tissues of infected trees. ACLSV was detected up to a dilution of 5(-5), whereas ApMV, ASGV, CVA, PPV and PNRSV up to 5(-4). The developed assay was validated following testing a total of 45 symptomatic leaf samples collected from different geographical regions of Jammu and Kashmir (India), and the presence of the viruses was further confirmed by RT-PCR and sequencing. The polyprobe, designed for performing molecular hybridization assay can be developed quickly and avoid the tedious transformation and cloning procedures. Apart from simultaneously detecting viruses in stone and pome fruit trees, it holds great potential for virus indexing programmes to ascertain the supply of virus-free plant materials to the growers.

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