Cereal Yellow Dwarf Virus-RPV (CYDV-RPV) ELISA Kit

Name: Cereal Yellow Dwarf Virus-RPV (CYDV-RPV) ELISA Kit
SKU: DEIAPV69
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

host plants

Intended Use

The test can be used to detect CYDV-rpv in infected barley, wheat and oats.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is moderately high. The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.

General Description

Barley yellow dwarf (BYD) is a worldwide virus disease of our most important grasses, including wheat, rice and maize. In the mid-1900s, the yellowing symptoms, transmission by aphids, and lack of transmission by rubbing (mechanical inoculation) differentiated BYD from many other plant virus diseases. BYD has served as a model for study of the "yellowing" virus diseases of plants and for elucidation of the mechanisms of circulative (also called persistent) virus transmission by aphids.
Barley yellow dwarf virus (BYDV) is a positive sense single-stranded RNA virus; the viron is not enveloped in a lipid coating. The virus is transmitted by aphids, and the taxonomy of the virus is based on genome organisation, serotype differences and on the primary aphid vector of each isolate.

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Screening the Spanish Barley Core Collection for disease resistance

PLANT BREEDING

Authors: Silvar, C.; Casas, A. M.; Kopahnke, D.; Habekuss, A.; Schweizer, G.; Gracia, M. P.; Lasa, J. M.; Ciudad, F. J.; Molina-Cano, J. L.; Igartua, E.; Ordon, F.

Abstract

The Spanish Barley Core Collection comprises 159 landrace-derived inbred lines and 16 cultivars adapted to Southern European conditions. The collection was screened for resistance to powdery mildew (Blumeria graminis), scald (Rhynchosporium secalis), leaf rust (Puccinia hordei), net blotch ( Pyrenophora teres f. teres), Barley yellow dwarf virus (BYDV) and Barley mild mosaic virus ( BaMMV). Resistance to powdery mildew was outstanding, with 58 lines presenting mean overall resistance, among them seven landrace-derived lines resistant to all seven isolates tested. About 26% of the Spanish lines were resistant to scald. Resistance to leaf rust and to net blotch was scarce, though a few accessions showed resistance levels as good as the checks. Thirteen accessions (12 Spanish) were totally resistant to BaMMV and c. 20% of accessions showed moderate tolerance to BYDV. Landrace-derived lines from the Mediterranean Coast and Southern regions of Spain were the most resistant to powdery mildew and leaf rust, but the most susceptible to viruses. Potential sources of resistance might be preserved in some accessions subjected to selective pressure in the region of origin.

Variation in barley yellow dwarf virus transmission efficiency by Rhopalosiphum padi (Homoptera : Aphididae) after acquisition from transgenic and nontransformed wheat genotypes

JOURNAL OF ECONOMIC ENTOMOLOGY

Authors: Jimenez-Martinez, ES; Bosque-Perez, NA

Abstract

The effects of different acquisition access periods (AAPs) and inoculation access periods (IAPs) on the transmission efficiency of barley yellow dwarf luteovirus (BYDV) by Rhopalosiplum padi (L.) (Honioptera: Aphididae) after fee ling on transgenic or nontransformed wheat, Triticum aestivum L., genotypes were studied. Three wheat genotypes were tested as virus sources: virus-susceptible 'Lambert' and 'Lanibert'-derived transgenic lines 103.1J and 126.02, which express the BYDV-PAV coat protein gene. Lower virus titers were measured in BYDV-infected transgenic plants compared with Lambert. No significant differences in transmission efficiency were detected for R. padi after varying IAPs. regardless of genotype. Transmission efficiency increased with an increase in AAP in all genotypes tested. However, AAPs ranging from 6 to 48 h on Lambert resulted in significantly greater transmission efficiency than similar periods on transgenic 103.1J. Maximum transmission efficiency (70%) was observed after a 48-h AAP on Lambert, whereas the same AAP oil 103.1J and 126.02 resulted in a significantly lower transmission efficiency (57%). Contrasts were used to compare the rates of transmission and the theoretical maximum transmission percentage among the different wheat genotypes serving as virus sources. Both parameters were significantly different among genotypes, indicating that viral acquisition from each genotype resulted in a unique pattern of virus transmission by R. padi. The lowest rate of virus transmission after an AAP was observed on 103.1J compared with 126.02 or Lambert. This is likely associated with a lower virus titer in 103.1J. This is the first report of transgenic virus resistance in wheat affecting the transmission efficiency of a virus vector.