Brocoli Necrotic yellow virus (BNYV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect BNYV in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.

General Description

Broccoli necrotic yellows virus (BNYV) is a plant pathogenic virus of the family Rhabdoviridae. BNYV had a limited host range by sap inoculation—3 of 17 plant species were infected including Datura stramonium from which it was characterized. The virus was not seed-borne, its longevity in vitro was between 1 and 3 days at 4 ° and less than 1 day at 23 °, its dilution end point was between 10−3 and 10−4, its thermal inactivation point between 45 ° and 50 °. BNYV was sensitive to ether indicating it contains lipid.

Standard Curve