BMX ELISA Kit (DEIA-XYA290)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cultured cells
Species Reactivity
Human, Mouse
Intended Use
The BAX Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor BAX protein expression profile in cells. The kit can be used for measuring the relative amounts of BAX in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on BAX.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-BAX Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months

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References


Chemical composition and structural characterization of contrasting colors of soybean seed coats

SEMINA-CIENCIAS AGRARIAS

Authors: Bahry, Carlos Andre; Acunha, Tanize dos Santos; Fernando, Juliana Aparecida; Chaves, Fabio Clasen; Nardino, Maicon; Zimmer, Paulo Dejalma

Seed coats provide protection to seeds and present differential traits between soybean genotypes. The aim of this study was to determine the chemical composition and analyze the internal coat structure of soybean genotypes with contrasting seed coat color, as well as to evaluate the rate of water absorption by seeds. Four genotypes were tested: two genotypes with black seed coat (IAC and TP lines) and two genotypes with yellow seed coat (BMX Potencia RR and CD 202 cultivars). The seeds of the four genotypes were grown during the 2012/0213 crop season, in a greenhouse at the Embrapa Clima Temperado - Estacao Experimental Terras Baixas. After the harvest, the seeds were stored in controlled conditions for five months. After this period, concentration of phenolic compounds, antioxidant potential, anthocyanins, carotenoids, and lignin in seed coats were determined at the Universidade Federal de Pelotas. Internal coat structure was examined on anatomical slides and documented with image capturing software using a microscope at the Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo. Finally, water absorption of contrasting seeds was evaluated at 42 hours of imbibition. Experimental design was completely randomized with five replicates. Data were subjected to analysis of variance and the means were compared by Tukey's test, at 5% probability level. Coat structure was characterized by the image analysis. Imbibition data were subjected to regression analysis. The concentration of phenolic compounds, antioxidant potential, anthocyanins, carotenoids, and lignin are higher in black coated genotypes. Their palisade layer and hourglass cells are thicker and the former are more lignified in black seed coats compared to yellow seed coats. The water gain by the seeds is dependent on the conditions in which the seeds were formed and varied between genotypes, independent of coat coloration and lignin concentration.

Resonance Character of Copper/Silver/Gold Bonding in Small MoleculeMX (X=F, Cl, Br, CH3, CF3) Complexes

CHEMPHYSCHEM

Authors: Zhang, Guiqiu; Yue, Huanjing; Weinhold, Frank; Wang, Hui; Li, Hong; Chen, Dezhan

The resonance character of Cu/Ag/Au bonding is investigated in BMX (M=Cu, Ag, Au; X=F, Cl, Br, CH3, CF3; B=CO, H2O, H2S, C2H2, C2H4) complexes. The natural bond orbital/natural resonance theory results strongly support the general resonance-type three-center/four-electron (3c/4e) picture of Cu/Ag/Au bonding, B:MXB+M:X-, which mainly arises from hyperconjugation interactions. On the basis of such resonance-type bonding mechanisms, the ligand effects in the more strongly bound OCMX series are analyzed, and distinct competition between CO and the axial ligand X is observed. This competitive bonding picture directly explains why CO in OCAuCF3 can be readily replaced by a number of other ligands. Additionally, conservation of the bond order indicates that the idealized relationship b(BM)+b(MX)=1 should be suitably generalized for intermolecular bonding, especially if there is additional partial multiple bonding at one end of the 3c/4e hyperbonded triad.

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