Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The BAZ1A knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
BAZ1A; bromodomain adjacent to zinc finger domain, 1A; bromodomain adjacent to zinc finger domain protein 1A; ACF1; hACF1; WALp1; WCRF180; ACF1, drosophila, homolog of; ATP dependent chromatin remodelling protein; ATP utilizing chromatin assembly and remodeling factor 1; ATP-dependent chromatin-remodeling protein; ATP-utilizing chromatin assembly and remodeling factor 1; B930060C03; BAZ1A_HUMAN; BC065123; Bromodomain adjacent to zinc finger domain 1A; cbp146; CHRAC subunit ACF1; DKFZp586E0518; FLJ14383; Gtl5; hWALp1; Williams syndrome transcription factor related chromatin remodeling factor 180; Williams syndrome transcription factor-related chromatin-remodeling factor 180; OTTHUMP00000178813; OTTHUMP00000244406; ATP-dependent chromatin remodeling protein; ATP-utilizing chromatin assembly and remodeling fac
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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