Total Antioxidant Power Colorimetric Microplate Assay (DEIA-XYL3)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
tissue lysate, cell culture, urine, plasma, food
Contents of Kit
1. DILUTION BUFFER: 60 mL of dilution buffer for the dilution of both sample and standard.
2. COPPER SOLUTION: 5 mL of Cu++ Solution.
3. STOP SOLUTION: 5 mL of stop solution.
4. TROLOX STANDARD: 1 lyophilized vial of 2 mM Trolox standard.
5. MICROPLATE: One optically clear 96 well microplate.
Storage
Store the components of this kit at the temperatures specified on the labels. Aliquot the unused portion of the reconstituted TROLOX STANDARD and store at -80C until kit expiration.
General Description
Oxidative stress has been implicated in a number of diseases such as atherosclerosis1, chronic inflammatory disease2, chronic renal failure3 and cancer4. Oxidative stress is the condition in which an imbalance between oxidant stimuli and physiological antioxidants exist leading to the damage of a cell. The body's physiological response to oxidative stress is through several antioxidant systems5 that include enzymes and varying sized molecules (see example below). These antioxidants can be found as watersoluble or lipid soluble molecules and localized transiently throughout tissues, cells and cell types.

Given the multiplicity of antioxidant pathways, their centrality in the prevention of oxidant stress, and the influences of lifestyle and nutritional supplements on an individual's antioxidant capacity, it is important to be able to quantitatively measure the total antioxidant capacity or antioxidant power within biological specimens6-11.

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