The Total Antibiotics in Milk Test Kit provides an easy and simple test method for detecting total antibiotics in milk. Compared with current commercially available kits, our kit is more rapid and simple to detect whether milk contains antibiotics at concentrations exceeding the Maximum Residue Limits (MRL). The MRL is required for government agencies, food manufacturers and processors, as well as quality assurance organizations in many countries.
The unique features of the kit are:
_ A quick spore growth assay (results can be read in about 3 hours)
_ Detection of wide range of antibiotics in milk
_ High sensitivity and low detection limit
_ High reproducibility
Contents of Kit
1. Return any unused plates/strips/wells to the plastic bag provided in the original package and reseal them. The shelf life is 6 months when the kit is properly stored.
2. Microtiter Plate: 1 x 96-well plates (8 wells x 12 strips), 2-8°C
3. Milk Negative Control Powder: 1 tube, Room Temperature
4. Milk Positive Control Powder: 1 tube, Room Temperature
5. Milk Negative Control Diluent: 1.5 mL, 2-8°C
6. Milk Positive Control Diluent**: 1.5 mL, -20°C
7. Aluminum Seal: 2 Sheets, Room Temperature
The method is based on the inhibition of a standard microbial growth recommended by international organizations. The kit is presented in a microplate format where each well contains medium agar spread with bacillus spores. When the plate (or a strip of wells) is incubated at 65°C , these bacillus spores germinate and the bacillus grows so that acids are produced, which result in the pH value changes. The color of the pH indicator in the medium changes from purple to yellow. If milk samples contain antibiotics at higher concentration than the detection limit, the bacillus will not grow and therefore the medium color remains unchanged.