Total Antibiotics Test Kit (DIA6309-1)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
milk
Species Reactivity
N/A
Intended Use
The Total Antibiotics in Milk Test Kit provides an easy and simple test method for detecting total antibiotics in milk. Compared with current commercially available kits, our kit is more rapid and simple to detect whether milk contains antibiotics at concentrations exceeding the Maximum Residue Limits (MRL). The MRL is required for government agencies, food manufacturers and processors, as well as quality assurance organizations in many countries.
The unique features of the kit are:
_ A quick spore growth assay (results can be read in about 3 hours)
_ Detection of wide range of antibiotics in milk
_ High sensitivity and low detection limit
_ High reproducibility
Contents of Kit
1. Return any unused plates/strips/wells to the plastic bag provided in the original package and reseal them. The shelf life is 6 months when the kit is properly stored.
2. Microtiter Plate: 1 x 96-well plates (8 wells x 12 strips), 2-8°C
3. Milk Negative Control Powder: 1 tube, Room Temperature
4. Milk Positive Control Powder: 1 tube, Room Temperature
5. Milk Negative Control Diluent: 1.5 mL, 2-8°C
6. Milk Positive Control Diluent**: 1.5 mL, -20°C
7. Aluminum Seal: 2 Sheets, Room Temperature
Storage
2-8°C or -20°C
Sensitivity
General Description
The method is based on the inhibition of a standard microbial growth recommended by international organizations. The kit is presented in a microplate format where each well contains medium agar spread with bacillus spores. When the plate (or a strip of wells) is incubated at 65°C , these bacillus spores germinate and the bacillus grows so that acids are produced, which result in the pH value changes. The color of the pH indicator in the medium changes from purple to yellow. If milk samples contain antibiotics at higher concentration than the detection limit, the bacillus will not grow and therefore the medium color remains unchanged.

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References


Tryptic Stability and Antimicrobial Activity of the Derivatives of Polybia-CP with Fine-Tuning Modification in the Side Chain of Lysine

INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS

Authors: Jia, Fengjing; Liang, Xiaolei; Wang, Jiayi; Zhang, Lishi; Zhou, Jingjing; He, Yuhang; Zhang, Fangfang; Yan, Wenjin; Wang, Kairong

Antimicrobial peptides (AMPs) were believed to be a class of promising antimicrobials to combat the increasing resistant microbes. However, AMPs could be easily degraded by endogenous proteases, which limited their clinic use. Considering that lysine is the only one cationic amino acid residue in polybia-CP which were related to the cleavage of trypsin like serine protease's digestion, we introduced lysine's mimics which kept the key function group side chain -NH2 and only varied the methylene chains in the sequence of polybia-CP to investigate the effect of the introduction of non-proteinogenic amino acids on the activity and stability of antimicrobial peptides. In addition, two analogs in which lysine were substituted by the other two proteinogenic cationic amino acids arginine and histidine also were synthesized to evaluate the effect of fine tuning the function group in lysine on its antimicrobial activity and stability. We found that the introduction of amino acids with shortened side chain length of lysine could enhance the trypsin resistance of the derivatives of polybia-CP, while maintain the same or comparable antimicrobial activity with the parent peptide. In addition, while lysine was substituted by histidine, His-CP demonstrated comparable antimicrobial activity and dramatically improved trypsin resistance. Although the analog exhibited excellent antimicrobial activity while lysine was substituted by Arginine residue (Arg-CP), its enzymatic stability was almost the same as its parent peptide. These results suggest that the fine-tuning of side chain of lysine may offer a promising strategy to improve the tryptic stability and retain the antimicrobial activity of antimicrobial peptide.

Molecular epidemiology of nonpharyngeal group A Streptococci isolates in northern Lebanon

FUTURE MICROBIOLOGY

Authors: Rafei, Rayane; Hawli, Malaik; Osman, Marwan; Khelissa, Simon; Salloum, Tamara; Dabboussi, Fouad; Tokajian, Sima; Hamze, Monzer

Aim: To characterize the epidemiology of group A Streptococcus (GAS) involved in nonpharyngeal infections sparingly addressed in Lebanon. Materials & methods: A collection of 63 nonpharyngeal GAS isolates recovered between 2010 and 2019 from northern Lebanon were analyzed through emm typing, virulence gene profiling, FCT typing and antibiotic susceptibility analysis. Results & conclusion: A total of 29 emm subtypes was detected, with emm1 being the most dominant. A great intraclonal divergence driven by the loss and gain of superantigens or by the structural variability within the FCT regions was unraveled. The resistance rates for erythromycin and tetracycline were 8 and 20.6%, respectively. The 30-valent vaccine coverage was 76%. This study evidences the complexity of the neglected GAS pathogen in Lebanon.

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