Anti-ACTA1 monoclonal antibody (DCABH-222)

Rabbit Anti-ACTA1 monoclonal antibody for WB, ICC/IF, IHC, IP Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
KK10-30
Species Reactivity
Human, Mouse, Rat, zebrafish
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:2,000; ICC: 1:100-1:500; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish; Lane 2: Hela Lane 3: A431; Lane 4: Jurkat
Fig2: ICC staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.

Target


Alternative Names
a actin; ACTA; ACTA1; Actin alpha skeletal muscle; Actin; actin, alpha 1, skeletal muscle 1
Entrez Gene ID

Citations


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References


Stern-Straeter, J; Bonaterra, GA; et al. Characterization of human myoblast differentiation for tissue-engineering purposes by quantitative gene expression analysis. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 5:E197-E206(2011).
Copeland, O; Nowak, KJ; et al. Investigation of changes in skeletal muscle alpha-actin expression in normal and pathological human and mouse hearts. JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY 31:207-214(2010).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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