Mouse anti-Human Macrophage surface antigen monoclonal antibody for IHC
Immunohistochemistry (10 μg/mL) & The optimal working dilution should be determined by the end user.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
AM-3K, was produced by using human alveolar macrophages as immunogen. BN-4Lreacts intensely with most of macrophages in lymphoreticular organs and in many other organs and tissues. BN-4Lalso reacts with the macrophages in many pathological conditions. However, this antibody does not react with dendritic cell population, such as epidermal Langerhans cells, interdigitating cells in the paracortex of lymph nodes, nor follicular dendritic cells. Lymphocytes, granulocytes and freshly isolated blood monocytes are also negative. Reaction products forBN-4Lwere found on the cytoplasmic membrane of macrophages by immunoelectron microscopy.
Poderoso, T; Martinez, P; et al. Delivery of antigen to sialoadhesin or CD163 improves the specific immune response in pigs. VACCINE 29:4813-4820(2011).
Kaur, R; Henriksen-Lacey, M; et al. Effect of Incorporating Cholesterol into DDA:TDB Liposomal Adjuvants on Bilayer Properties, Biodistribution, and Immune Responses. MOLECULAR PHARMACEUTICS 11:197-207(2014).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.