Anti-ZP2 monoclonal antibody (DCABH-684)

Mouse anti-Human ZP2 monoclonal antibody for WB Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2a
Species Reactivity
Human
Immunogen
Recombinant fragment, corresponding to amino acids 400-510 of Human ZP2 with a Proprietary tag (NP_003451).
Conjugate
Unconjugated

Applications


Application Notes
WB: 1 - 5 μg/ml;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
ZP2; zona pellucida glycoprotein 2 (sperm receptor); zona pellucida sperm-binding protein 2; zona pellucida protein A; zona pellucida glycoprotein ZP2; ZPA
Entrez Gene ID
UniProt ID

Product Background


Gene summary
ZP2 (Zona Pellucida Glycoprotein 2) is a Protein Coding gene. Diseases associated with ZP2 include infertility and acheiropody. Among its related pathways are Reproduction and Ovarian Infertility Genes. GO annotations related to this gene include coreceptor activity and acrosin binding. An important paralog of this gene is ZP1. The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants.
Antigen Description
The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three (mouse) or four (human) glycoproteins (ZP1-4) with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.
Pathway
Ovarian Infertility Genes, organism-specific biosystem.

Citations


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References


Lea, IA; Widgren, EE; et al. Analysis of recombinant mouse zona pellucida protein 2 (ZP2) constructs for immunocontraception. VACCINE 20:1515-1523(2002).
Kerr, CL; Hanna, WF; et al. Characterization of zona pellucida glycoprotein 3 (ZP3) and ZP2 binding sites on acrosome-intact mouse sperm. BIOLOGY OF REPRODUCTION 66:1585-1595(2002).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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