Human Anti-Vulvar Cancer Hybridoma [WMO7I3] (CSC-H0710)

This hybridoma produces mAbs (IgG) against Vulvar Cancer Datasheet

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General Information


Isotype
IgG
Fusion Species
Human X Human Hybridoma
Immunological Donor
Human B lymphocytes
Clone
WMO7I3
Cell Line Description
The fusion line is reported to be EBNA positive. Lymphocytes were isolated from a regional draining lymph node of a patient with carcinoma of the vulva. B lymphocytes were fused with UC 729-6 cells . The antibody recognizes an epitope on a 78000 dalton antigen on A-431 cells and a 66000 dalton antigen on T293H cells.
Morphology
Lymphoblast
Growth Properties
Suspension

Culture Method


Complete Growth Medium
RPMI 1640 with 2 mM L-glutamine, supplemented with 10% FBS
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 2x10^5-8x10^5 cells/ml
Storage
Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO

Target


Introduction
Vulvar cancer, a malignant invasive growth in the vulva, accounts for about 4 % of all gynecological cancers and typically affects women in later life. It is estimated that in the United States in 2006 about 3, 740 new cases will be diagnosed and about 880 women will die as a result of vulvar cancer Vulvar carcinoma is separated from vulvar intraepithelial neoplasia (VIN), a non-invasive lesion of the epithelium that can progress via carcinoma-in-situ to squamous cell cancer, and from Paget disease of the vulva.

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