GeneralInformation
Immunogen
Vesicular stomatitis virus, Indiana Serotype (VSV-Ind.)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with vesicular stomatitis virus, Indiana Serotype (VSV-Ind.). Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind.).
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium(45%heat-inactivated horse serum), add 10% (v/v) DMSO
Citations
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Ito, H; Watanabe, S; et al. Ebola virus glycoprotein: Proteolytic processing, acylation, cell tropism, and detection of neutralizing antibodies. JOURNAL OF VIROLOGY 75:1576-1580(2001).
van Koningsbruggen, S; Straasheijm, KR; et al. FRG1P-mediated aggregation of proteins involved in pre-mRNA processing. CHROMOSOMA 116:53-64(2007).
COA
Certificate of Analysis
