Anti-V5 tag polyclonal antibody (DPATB-H83311)

Rabbit anti-V5 tag polyclonal antibody for ICC/IF, ChIP, WB, ELISA, IP, ChIP/Chip, ICC

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
N/A
Immunogen
GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.
Conjugate
Unconjugated

Target


Alternative Names
GKPIPNPLLGLDST epitope tag,GKPIPNPLLGLDST tag,Protein Rev,Regulator of expression of viral proteins,rev,V5 epitope tag,

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References


A stealth adhesion factor contributes to Vibrio vulnificus pathogenicity: Flp pili play roles in host invasion, survival in the blood stream and resistance to complement activation

PLOS PATHOGENS

Authors: Tra-My Duong-Nu; Jeong, Kwangjoon; Hong, Seol Hee; Puth, Sao; Kim, Soo Young; Tan, Wenzhi; Lee, Kwang Ho; Lee, Shee Eun; Rhee, Joon Haeng

The tad operons encode the machinery required for adhesive Flp (fimbrial low-molecular-weight protein) pili biogenesis. Vibrio vulnificus, an opportunistic pathogen, harbors three distinct tad loci. Among them, only tad1 locus was highly upregulated in in vivo growing bacteria compared to in vitro culture condition. To understand the pathogenic roles of the three tad loci during infection, we constructed single, double and triple tad loci deletion mutants. Interestingly, only the Delta tad123 triple mutant cells exhibited significantly decreased lethality in mice. Ultrastructural observations revealed short, thin filamentous projections disappeared on the.tad123 mutant cells. Since the pilin was paradoxically non-immunogenic, a V5 tag was fused to Flp to visualize the pilin protein by using immunogold EM and immunofluorescence microscopy. The Delta tad123 mutant cells showed attenuated host cell adhesion, decreased biofilm formation, delayed RtxA1 exotoxin secretion and subsequently impaired translocation across the intestinal epithelium compared to wild type, which could be partially complemented with each wild type operon. The Delta tad123 mutant was susceptible to complement-mediated bacteriolysis, predominantly via the alternative pathway, suggesting stealth hiding role of the Tad pili. Complement depletion by treating with anti-C5 antibody rescued the viable count of Delta tad123 in infected mouse bloodstream to the level comparable to wild type strain. Taken together, all three tad loci cooperate to confer successful invasion of V. vulnificus into deeper tissue and evasion from host defense mechanisms, ultimately resulting in septicemia.

Systematic screen for Drosophila transcriptional regulators phosphorylated in response to insulin/mTOR pathway

G3-GENES GENOMES GENETICS

Authors: Liu, Ying; Mattila, Jaakko; Hietakangas, Ville

Insulin/insulin-like growth factor signaling (IIS) is a conserved mechanism to regulate animal physiology in response to nutrition. IIS activity controls gene expression, but only a subset of transcriptional regulators (TRs) targeted by the IIS pathway is currently known. Here we report the results of an unbiased screen forDrosophilaTRs phosphorylated in an IIS-dependent manner. To conduct the screen, we built a library of 857 V5/Strep-tagged TRs under the control of Copper-inducible metallothionein promoter (pMt). The insulin-induced phosphorylation changes were detected by using Phos-tag SDS-PAGE and Western blotting. Eight proteins were found to display increased phosphorylation after acute insulin treatment. In each case, the insulin-induced phosphorylation was abrogated by mTORC1 inhibitor rapamycin. The hits included two components of the NURF complex (NURF38 and NURF55), bHLHZip transcription factor Max, as well as theDrosophilaortholog of human proliferation-associated 2G4 (dPA2G4). Subsequent experiments revealed that the expression of thedPA2G4gene was promoted by the mTOR pathway, likely through transcription factor Myc. Furthermore, NURF38 was found to be necessary for growth in larvae, consistent with the role of IIS/mTOR pathway in growth control.

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